Chemistry is the experimental science by definition. We want to make observations to prove hypothesis. For this purpose, we perform experiments in the lab. , Category: amides-buliding-blocks, 4316-74-9, Name is Sodium 2-(methylamino)ethanesulfonate, molecular formula is C3H8NNaO3S, belongs to amides-buliding-blocks compound. In a document, author is Mikhailovskii, A. G., introduce the new discover.
Influence of UV radiation on molecular structure and catalytic activity of free and immobilized bromelain, ficin and papain
Our research has shown that the degree of photosensitivity of the cysteine proteases can be arranged in the following order: bromelain -> ficin -> papain. After the UV irradiation with 151 J.m(-2) intensity of a bromelain solution, the enzyme activity has increased. No decrease in the catalytic capacity and the change in the size of the molecule was recorded in the 151-6040 J.m(-2) range of irradiation intensities. A decrease in the catalytic capacity of ficin and the increase of its globule size occurred after exposure to a radiation of 3020 J.m(-2) intensity. The decrease in papain activity was observed at the UV irradiation intensity of 453 J.m(-2), and an increase of the papain globule size was detected at 755 J.m(-2). Immobilization on chitosan matrix leads to the increase in the stability of heterogeneous biocatalysts with respect to UV irradiation in comparison with free enzymes. The changes in IR spectra of immobilized cysteine proteases practically do not affect the bands due to the protein component of the system: amide I, amide II, amide III. Therefore, it can be postulated that the chitosan matrix acts as photoprotector for immobilized ficin, bromelain and papain. The obtained results can be helpful for development of drugs based on chitosan and cysteine proteases in combination with phototherapy, as well as for choosing their sterilization conditions.
A reaction mechanism is the microscopic path by which reactants are transformed into products. Each step is an elementary reaction. In my other articles, you can also check out more blogs about 4316-74-9. Category: amides-buliding-blocks.