In 2018,Xin, Bo-Tao; van Tol, Bianca D. M.; Ovaa, Huib; Geurink, Paul P. published 《Native chemical ligation at methionine bioisostere norleucine allows for N-terminal chemical protein ligation》.Organic & Biomolecular Chemistry published the findings.Computed Properties of C11H24N2O The information in the text is summarized as follows:
The development of γ-thionorleucine (ThioNle) as a handle for native chem. ligation-desulfurization is reported here. ThioNle is a new addition to the expanding thiolated amino acid toolbox and serves as a methionine substitute in NCL with the advantage that it lacks the undesirable oxidation-prone thioether moiety. Its usefulness for N-terminal ubiquitination is demonstrated by efficient preparation of fully synthetic linear diubiquitin with preserved protein folding compared to the expressed material. Interestingly, gel-based deubiquitinating assays revealed that the methionine to norleucine substitution did affect diubiquitin cleavage, which may indicate a more profound role for methionine in the interaction between ubiquitin and the deubiquitinating enzymes than has been known so far. In the experiment, the researchers used tert-Butyl N,N’-diisopropylcarbamimidate(cas: 71432-55-8Computed Properties of C11H24N2O)
tert-Butyl N,N’-diisopropylcarbamimidate(cas: 71432-55-8) belongs to anime. Reaction with nitrous acid (HNO2), which functions as an acylating agent that is a source of the nitrosyl group (―NO), converts aliphatic primary amines to nitrogen and mixtures of alkenes and alcohols corresponding to the alkyl group in a complex process. This reaction has been used for analytical determination of primary amino groups in a procedure known as the Van Slyke method.Computed Properties of C11H24N2O
Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics