Mut, Ludmila; Grobosch, Thomas; Binscheck-Domass, Torsten; Frenzel, Wolfgang published an article in 2016, the title of the article was Toxicological screening of human plasma by on-line SPE-HPLC-DAD: identification and quantification of acidic and neutral drugs.Category: amides-buliding-blocks And the article contains the following content:
A multi-analyte screening method for the quantification of 50 acidic/neutral drugs in human plasma based on online solid-phase extraction (SPE)-HPLC with photodiode array detection (DAD) was developed, validated and applied for clin. investigation. Acetone and methanol for protein precipitation, three different SPE materials (two electro-neutral, one strong anion-exchange, one weak cation-exchange) for online extraction, five HPLC-columns [one C18 (GeminiNX), two phenyl-hexyl (Gemini C6-Ph, Kinetex Phenyl-Hexyl) and two pentafluorophenyl (LunaPFP(2), KinetexPFP)] for anal. separation were tested. For sample pre-treatment, acetone in the ratio 1:2 (plasma:acetone) showed a better baseline and fewer matrix peaks in the chromatogram than methanol. Only the strong anion-exchanger SPE cartridge (StrataX-A, pH 6) allowed the extraction of salicylic acid. Anal. separation was carried out on a Gemini C6-Ph column (150 × 4.6 mm, 3 μm) using gradient elution with acetonitrile-water 90:10 (volume/volume) and phosphate buffer (pH 2.3). Linear calibration curves with correlation coefficients r ≥ 0.9950/0.9910 were obtained for 46/four analytes. Addnl., this method allows the quantification of 23 analytes for therapeutic drug monitoring. Limits of quantitation ranged from 0.1 (amobarbital) to 23 mg/L (salicylic acid). Inter-/intra-day precisions of quality control samples (low/high) were better than 13% and accuracy (bias) ranged from -14 to 10%. A computer-assisted database was created for automated detection of 223 analytes of toxicol. interests. Four cases of multi-drug intoxications are presented. Copyright © 2015 John Wiley & Sons, Ltd. The experimental process involved the reaction of 2-(4-Methylbenzamido)acetic acid(cas: 27115-50-0).Category: amides-buliding-blocks
The Article related to drug toxicol screening human plasma spe hplc dad, acidic and neutral drugs, multi-analyte screening, on-line solid-phase extraction, plasma samples, systematic toxicological analysis, validation and other aspects.Category: amides-buliding-blocks
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