On May 31, 2021, Kuljanin, Miljan; Mitchell, Dylan C.; Schweppe, Devin K.; Gikandi, Ajami S.; Nusinow, David P.; Bulloch, Nathan J.; Vinogradova, Ekaterina V.; Wilson, David L.; Kool, Eric T.; Mancias, Joseph D.; Cravatt, Benjamin F.; Gygi, Steven P. published an article.Related Products of 79-07-2 The title of the article was Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. And the article contained the following:
Current methods used for measuring amino acid side-chain reactivity lack the throughput needed to screen large chem. libraries for interactions across the proteome. Here we redesigned the work flow for activity-based protein profiling of reactive cysteine residues by using a smaller desthiobiotin-based probe, sample multiplexing, reduced protein starting amounts and software to boost data acquisition in real time on the mass spectrometer. Our method, streamlined cysteine activity-based protein profiling (SLC-ABPP), achieved a 42-fold improvement in sample throughput, corresponding to profiling library members at a depth of >8,000 reactive cysteine sites at 18 min per compound We applied it to identify proteome-wide targets of covalent inhibitors to mutant Kirsten rat sarcoma (KRAS)G12C and Bruton’s tyrosine kinase (BTK). In addition, we created a resource of cysteine reactivity to 285 electrophiles in three human cell lines, which includes >20,000 cysteines from >6,000 proteins per line. The goal of proteome-wide profiling of cysteine reactivity across thousand-member libraries under several cellular contexts is now within reach. The experimental process involved the reaction of 2-Chloroacetamide(cas: 79-07-2).Related Products of 79-07-2
The Article related to reactive cysteine screening large electrophile library, Biochemical Methods: Biological and other aspects.Related Products of 79-07-2
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Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics