Dijkman, R. published the artcileAn affinity column for phospholipase A2 based on immobilized acylaminophospholipid analogs, HPLC of Formula: 1869-45-0, the publication is Biochimica et Biophysica Acta, Lipids and Lipid Metabolism (1997), 1347(1), 1-8, database is CAplus and MEDLINE.
A synthetic route was developed to prepare 2-acylamino phospholipid analogs suitable for immobilization. The inhibitors, synthesized in either the (R)- and (S)-configuration, carried an ω-carboxyl group in one acyl chain for immobilization to the matrix. As a matrix Sepharose 6B, derivatized with a polar, non-charged 16 atom spacer was used. Low-mol. weight phospholipase A2 binds in a calcium-dependent way to the immobilized (S)-inhibitor and not to the immobilized (R)-inhibitor which shows that binding involves specific active site interactions rather than hydrophobic chromatog. The specificity was further demonstrated by the fact that the immobilized (S)-inhibitor binds porcine pancreatic and snake venom phospholipases A2, but not the porcine pancreatic zymogen. Moreover, a mutant porcine pancreatic phospholipase A2 in which the active side residue His48 has been replaced by Gln, was not bound by the column. This column material might be applicable for affinity purification of phospholipase A2 and for screening of phage display libraries.
Biochimica et Biophysica Acta, Lipids and Lipid Metabolism published new progress about 1869-45-0. 1869-45-0 belongs to amides-buliding-blocks, auxiliary class Trifluoromethylated Building Blocks, name is 2,2,2-Trifluoro-N-(2-mercaptoethyl)acetamide, and the molecular formula is C4H6F3NOS, HPLC of Formula: 1869-45-0.
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