Multispectral autofluorescence characteristics of reproductive aging in old and young mouse oocytes was written by Campbell, Jared M.;Mahbub, Saabah B.;Bertoldo, Michael J.;Habibalahi, Abbas;Goss, Dale M.;Ledger, William L.;Gilchrist, Robert B.;Wu, Lindsay E.;Goldys, Ewa M.. And the article was included in Biogerontology in 2022.HPLC of Formula: 1094-61-7 The following contents are mentioned in the article:
Abstract: Increasing age has a major detrimental impact on female fertility, which, with an aging population, has major sociol. implications. This impact is primarily mediated through deteriorating quality of the oocyte. Deteriorating oocyte quality with biol. age is the greatest rate-limiting factor to female fertility. Here we have used label-free, non-invasive multi-spectral imaging to identify unique autofluorescence profiles of oocytes from young and aged animals. Discriminant anal. demonstrated that young oocytes have a distinct autofluorescent profile which accurately distinguishes them from aged oocytes. We recently showed that treatment with the NAD (NAD+) precursor NMN (NMN) restored oocyte quality and fertility in aged animals, and when our anal. was applied to oocytes from aged animals treated with NMN, 85of these oocytes were classified as having the autofluorescent signature of young animals. Spectral unmixing using the Robust Dependent Component Anal. (RoDECA) algorithm demonstrated that NMN treatment altered the metabolic profile of oocytes, increasing free NAD(P)H, protein bound NAD(P)H, redox ratio and the ratio of bound to free NAD(P)H. The frequency of oocytes with simultaneously high NAD(P)H and flavin content was also significantly increased in mice treated with NMN. Young and Aged + NMN oocytes had a smoother spectral distribution, with the distribution of NAD(P)H in young oocytes specifically differing from that of aged oocytes. Identifying the multispectral profile of oocyte autofluorescence during aging could have utility as a non-invasive and sensitive measure of oocyte quality. This study involved multiple reactions and reactants, such as ((2R,3S,4R,5R)-5-(3-Carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl hydrogen phosphate (cas: 1094-61-7HPLC of Formula: 1094-61-7).
((2R,3S,4R,5R)-5-(3-Carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl hydrogen phosphate (cas: 1094-61-7) belongs to amides. Compared to amines, amides are very weak bases and do not have clearly defined acid–base properties in water. On the other hand, amides are much stronger bases than esters, aldehydes, and ketones. Amides can be freed from solvent or water by drying below their melting points. These purifications can also be used for sulfonamides and acid hydrazides.HPLC of Formula: 1094-61-7
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