Advancing HPLC-PDA-HRMS-SPE-NMR Analysis of Coumarins in Coleonema album by Use of Orthogonal Reversed-Phase C18 and Pentafluorophenyl Separations was written by Lima, Rita de Cassia L.;Gramsbergen, Simone M.;Van Staden, Johannes;Jager, Anna K.;Kongstad, Kenneth T.;Staerk, Dan. And the article was included in Journal of Natural Products in 2017.Name: (2E,4E)-N-Isobutyldeca-2,4-dienamide The following contents are mentioned in the article:
A hyphenated procedure involving high-performance liquid chromatog., photodiode array detection, high-resolution mass spectrometry, solid-phase extraction, and NMR spectroscopy, i.e., HPLC-PDA-HRMS-SPE-NMR, has proven an effective technique for the identification of compounds in complex matrixes. Most HPLC-PDA-HRMS-SPE-NMR investigations reported so far have relied on anal.-scale reversed-phase C18 columns for separation Herein is reported the use of an anal.-scale pentafluorophenyl column as an orthogonal separation method following fractionation of a crude Et acetate extract of leaves of Coleonema album on a preparative-scale C18 column. This setup allowed the HPLC-PDA-HRMS-SPE-NMR anal. of 23 coumarins, including six new compounds, 8-O-β-
(2E,4E)-N-Isobutyldeca-2,4-dienamide (cas: 18836-52-7) belongs to amides. Amides can be viewed as a derivative of a carboxylic acid RC(=O)OH with the hydroxyl group –OH replaced by an amine group −NR′R″; or, equivalently, an acyl (alkanoyl) group RC(=O)− joined to an amine group. Amides can be recrystallised from large quantities of water, ethanol, ethanol/ether, aqueous ethanol, chloroform/toluene, chloroform or acetic acid. The likely impurities are the parent acids or the alkyl esters from which they have been made. The former can be removed by thorough washing with aqueous ammonia followed by recrystallisation, whereas elimination of the latter is by trituration or recrystallisation from an organic solvent.Name: (2E,4E)-N-Isobutyldeca-2,4-dienamide
Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics