Simultaneous determination of six marker compounds in Piper nigrum L. and species comparison study using high-performance thin-layer chromatography-mass spectrometry was written by Ramesh, Bokka;Sarma, Vanka Uma Maheswara;Kumar, Katragunta;Babu, Katragadda Suresh;Devi, Potturi Sita. And the article was included in Journal of Planar Chromatography–Modern TLC in 2015.Electric Literature of C14H25NO The following contents are mentioned in the article:
The isolation and characterization of bioactive compounds from medicinal plants is usually a significant challenge in phytochem. anal. because of the natural chem. complexity of plant extracts However, there exists a need for anal. tools which can quant. sep. and characterize the components from these biosources with greater chromatog. selectivity and lesser anal. run times that facilitate the evaluation with enhanced separation profiles. Hyphenation of thin-layer chromatog. (TLC/HPTLC) with mass spectrometry (MS) is an alternative for screening herbal extracts because of its rapid anal. and ability to aid structural characterization with powerful anal. capacity. The aim of the present study was to develop a sophisticated anal. method which utilizes HPTLC-MS coupling for the chromatog. profiling and evaluation of the therapeutically important genus Piper (Piperaceae). In this study, six marker compounds, namely, trichostachine, piperine, 4,5-dihydropiperlonguminine, guineensine, pellitorine, and sesamin were analyzed and quantified in extracts of Piper nigrum L. and compared with those of Piper longum L. and Piper chaba Hunter. All the samples tested showed similar phytochem. profiles, but the contents of the active ingredients varied. Addnl., HPTLC-MS further allowed confirming the identification of the constituents in the analyzed samples with greater chromatog. selectivity where HPTLC facilitated a selective chromatog. resolution, while MS offered an efficient characterization of the target compounds in one anal. run. The study finds a potential utility in adopting HPTLC-MS as a rapid and high throughput method for the efficient quantification and identification of marker compounds from medicinal plants. This study involved multiple reactions and reactants, such as (2E,4E)-N-Isobutyldeca-2,4-dienamide (cas: 18836-52-7Electric Literature of C14H25NO).
(2E,4E)-N-Isobutyldeca-2,4-dienamide (cas: 18836-52-7) belongs to amides. Because of the greater electronegativity of oxygen, the carbonyl (C=O) is a stronger dipole than the N–C dipole. The presence of a C=O dipole and, to a lesser extent a N–C dipole, allows amides to act as H-bond acceptors. The presence of the amide group –C(=O)N– is generally easily established, at least in small molecules. It can be distinguished from nitro and cyano groups in IR spectra. Amides exhibit a moderately intense νCO band near 1650 cm−1. By 1H NMR spectroscopy, CONHR signals occur at low fields. In X-ray crystallography, the C(=O)N center together with the three immediately adjacent atoms characteristically define a plane.Electric Literature of C14H25NO
Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics