Long-chain fatty acids regulate SIRT3 expression by affecting intracellular NAD+ levels in large yellow croaker (Larimichthys crocea) was written by Chen, Qiang;Liu, Qiangde;Hao, Tingting;Cui, Kun;Zhao, Zengqi;Mai, Kangsen;Ai, Qinghui. And the article was included in Aquaculture in 2022.SDS of cas: 1094-61-7 The following contents are mentioned in the article:
Sirtuin 3 (SIRT3) is a major deacetylase in the mitochondria and sensitive to diverse nutrient signals. However, how dietary fatty acids regulate SIRT3 expression remains poorly understood. Here, sirt3 gene from large yellow croaker was characterized and can be activated by the oxidized NAD (NAD+) precursor, NMN (NMN). Moreover, the expression of SIRT3 is regulated differently by dietary fatty acids. In vivo, soybean oil increased the mRNA expression of sirt3 in head kidney. In vitro, stearic acid (SA) decreased the expression of SIRT3 in macrophages, while DHA and EPA increased the mRNA expression of sirt3. Meanwhile, all long-chain polyunsaturated fatty acids (LC-PUFAs) enhanced the protein levels of SIRT3, which was consistent with the trend of fatty acids affecting NAD+ levels. Furthermore, inhibition of NAD+de novo synthesis blocked DHA-induced increases in SIRT3 expression, and NMN supplement reversed SA-induced decreases in SIRT3 expression. In conclusion, these findings suggest that dietary fatty acids may regulate SIRT3 expression by affecting intracellular NAD+ synthesis. Regarding the important role of SIRT3 in regulating mitochondrial function, appropriate activation of SIRT3 may be an important way to alleviate metabolic disorders in aquaculture. This study involved multiple reactions and reactants, such as ((2R,3S,4R,5R)-5-(3-Carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl hydrogen phosphate (cas: 1094-61-7SDS of cas: 1094-61-7).
((2R,3S,4R,5R)-5-(3-Carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl hydrogen phosphate (cas: 1094-61-7) belongs to amides. In primary and secondary amides, the presence of N–H dipoles allows amides to function as H-bond donors as well. Thus amides can participate in hydrogen bonding with water and other protic solvents; the oxygen atom can accept hydrogen bonds from water and the N–H hydrogen atoms can donate H-bonds. Amides can be recrystallised from large quantities of water, ethanol, ethanol/ether, aqueous ethanol, chloroform/toluene, chloroform or acetic acid. The likely impurities are the parent acids or the alkyl esters from which they have been made. The former can be removed by thorough washing with aqueous ammonia followed by recrystallisation, whereas elimination of the latter is by trituration or recrystallisation from an organic solvent.SDS of cas: 1094-61-7
Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics