Cytotoxicity evaluation and characterization of chloroform extract of leaf of Piper sarmentosum possessing antiangiogenic activity was written by Hussain, Khalid;Ismail, Zhari;Sadikun, Amirin;Ibrahim, Pazillah. And the article was included in Pharmacologyonline in 2009.Application of 18836-52-7 The following contents are mentioned in the article:
The chloroform extract of leaves of Piper sarmentosum, a traditional medicinal plant, have shown promising antiangiogenic activity, IC50 45 μg/mL. Therefore, present study aimed to investigate the extract to rule out the probability of the involvement of cell death in the activity, and characterize the extract using colorimetry, gas chromatog. time of the flight mass spectrometry (GC-TOFMS) and high performance liquid chromatog. (HPLC). Different concentrations of the extract were evaluated by MTT cell-viability assay using human hepatic carcinoma cell line (HepG2) and human umbilical vascular endothelial cell line (HUVEC). Vincristine sulfate (IC50 0.016 μg/mL) was used as a pos. control. The extract exhibited IC50 76.24 μg/mL for HepG2 cells and 64.43 μg/mL for HUVEC. These IC50 values were found to be higher than IC50 (45 μg/mL) of the extract for antiangiogenic activity. The extract was analyzed for total amide content using colorimetric method and to characterize two main peaks by GC-TOFMS which were found to be pellitorine and sarmentine. In HPLC anal., the extract was found to have pellitorine (0.020 mg/g) and sarmentine (0.006 mg/g). Both of these markers exhibited 30 % antiangiogenic activity. The results of this study indicate that the extract inhibits angiogenesis without changing morphol. and viability of vascular endothelial cells. This study involved multiple reactions and reactants, such as (2E,4E)-N-Isobutyldeca-2,4-dienamide (cas: 18836-52-7Application of 18836-52-7).
(2E,4E)-N-Isobutyldeca-2,4-dienamide (cas: 18836-52-7) belongs to amides. The solubilities of amides and esters are roughly comparable. Typically amides are less soluble than comparable amines and carboxylic acids since these compounds can both donate and accept hydrogen bonds. Tertiary amides, with the important exception of N,N-dimethylformamide, exhibit low solubility in water. Ionic, or saltlike, amides are strongly alkaline compounds ordinarily made by treating ammonia, an amine, or a covalent amide with a reactive metal such as sodium.Application of 18836-52-7
Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics