Simultaneous determination of bioactive compounds in Piper nigrum L. and a species comparison study using HPLC-PDA was written by Rao, Vidadala Rama Subba;Raju, Sagi Satyanarayana;Sarma, Vanka Umamaheswara;Sabine, Fouriner;Babu, Kothapalli Hari;Babu, Katragadda Suresh;Rao, Janaswamy Madhusudana. And the article was included in Natural Product Research in 2011.Recommanded Product: 18836-52-7 The following contents are mentioned in the article:
Piper nigrum L. is a traditional medicine widely used in India for illnesses such as constipation, diarrhea, earache, gangrene, heart disease, hernia, hoarseness, indigestion, insect bites, insomnia, joint pain, liver problems, lung disease, oral abscesses, sunburn, tooth decay and toothaches. In this study, six bioactive compounds, namely piperine (1), pellitorine (2), guineensine (3), pipnoohine (4), trichostachine (5) and piperonal (6) were quantified in different extracts of P. nigrum L. and compared with those of P. longum L. and P. chaba Hunter. To evaluate the quality of P. nigrum, a simple, accurate and precise HPLC-PDA method was developed for the simultaneous determination of the above-mentioned six compounds The separation was achieved by Phenomenex Luna RP C18 column (150 × 4.6 mm, 5 μm, Phenomenex Inc, CA, USA) with a binary gradient solvent system of water-acetonitrile, at a flow rate of 1.0 mL min-1 and detected at 210, 232, 262 and 343 nm. All six calibration curves showed good linearity (R 2 > 0.9966). The method was reproducible with intra- and inter-day variations of less than 2% and 5%, resp. The results demonstrated that this method is simple, reliable and suitable for the quality control of these plants. This study involved multiple reactions and reactants, such as (2E,4E)-N-Isobutyldeca-2,4-dienamide (cas: 18836-52-7Recommanded Product: 18836-52-7).
(2E,4E)-N-Isobutyldeca-2,4-dienamide (cas: 18836-52-7) belongs to amides. Amides can be viewed as a derivative of a carboxylic acid RC(=O)OH with the hydroxyl group –OH replaced by an amine group −NR′R″; or, equivalently, an acyl (alkanoyl) group RC(=O)− joined to an amine group. The presence of the amide group –C(=O)N– is generally easily established, at least in small molecules. It can be distinguished from nitro and cyano groups in IR spectra. Amides exhibit a moderately intense νCO band near 1650 cm−1. By 1H NMR spectroscopy, CONHR signals occur at low fields. In X-ray crystallography, the C(=O)N center together with the three immediately adjacent atoms characteristically define a plane.Recommanded Product: 18836-52-7
Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics