Separation and purification of compounds from Piper kadsura using preparative reversed-phase liquid chromatography and preparative supercritical fluid chromatography was written by Xin, Huaxia;Peng, Ziyue;Jiang, Dasen;Fu, Qing;Jin, Yu;Liang, Xinmiao. And the article was included in Sepu in 2018.Formula: C14H25NO The following contents are mentioned in the article:
A method based on preparative reversed-phase liquid chromatog. (prep-RPLC) and preparative supercritical fluid chromatog. (prep-SFC) was developed for the separation and purification of compounds from piper kadsura. A pretreatment method was first developed, including methanol extraction, water precipitation, petroleum ether extraction, etc. Chlorophyll and other strong polar impurities were removed from the piper kadsura samples, and the target components were enriched in petroleum ether extracts The piper kadsura samples were separated into 18 fractions on a Unitary C18 column (250 mm × 20 mm, 5 μm) with water and methanol as the mobile phases. Then, the SFC parameters, including the column, modifier, temperature, and backpressure were optimized. The optimized conditions for prep-SFC were as follows: XAmide column (250 mm × 20 mm, 5 μm), methanol as the modifier, 30°C column temperature, and 15.0 MPa backpressure. Because of the good orthogonality of RPLC and SFC, six highly pure compounds were isolated, including kadsurenone, wallichinine, denudatin B, pellitorine, 2E-decenoic acid N-isobutylamide, and futoxide. This study involved multiple reactions and reactants, such as (2E,4E)-N-Isobutyldeca-2,4-dienamide (cas: 18836-52-7Formula: C14H25NO).
(2E,4E)-N-Isobutyldeca-2,4-dienamide (cas: 18836-52-7) belongs to amides. In primary and secondary amides, the presence of N–H dipoles allows amides to function as H-bond donors as well. Thus amides can participate in hydrogen bonding with water and other protic solvents; the oxygen atom can accept hydrogen bonds from water and the N–H hydrogen atoms can donate H-bonds. As a result of interactions such as these, the water solubility of amides is greater than that of corresponding hydrocarbons. These hydrogen bonds are also have an important role in the secondary structure of proteins.Formula: C14H25NO
Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics