Design of an in vitro multienzyme cascade system for the biosynthesis of nicotinamide mononucleotide was written by Zhou, Cailian;Feng, Jiao;Wang, Jing;Hao, Ning;Wang, Xin;Chen, Kequan. And the article was included in Catalysis Science & Technology in 2022.Application In Synthesis of ((2R,3S,4R,5R)-5-(3-Carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl hydrogen phosphate The following contents are mentioned in the article:
For the biosynthesis of NMN (NMN), three artificial pathways including the nicotinamide ribose phosphorylation pathway, adenosine phosphate pyrophosphorylation pathway, and adenosine phosphate hydrolysis (APH) pathway were designed and successfully conducted to produce NMN in vitro. The APH pathway, using AMP nucleosidase, ribose-phosphate diphosphokinase, and nicotinamide phosphoribosyltransferase (NAMPT), exhibited the highest level of NMN synthesis. To further improve NMN production via the APH pathway, various NAMPT orthologues were screened. The effects of temperature, pH, metal ions and enzyme ratios were further systematically investigated, and the accumulation of ADP was identified limiting pathway efficiency. Subsequently, an ATP recycling process was achieved by adding polyphosphate kinase 2 to convert ADP to ATP. With the optimized four multienzyme cascade catalysis systems, the NMN titer was increased to 9 mmol L-1 (3.0 g L-1) from 600μmol L-1 (0.2 g L-1) in vitro. This is the first study to use a multienzyme cascade catalysis process for NMN biosynthesis. This study involved multiple reactions and reactants, such as ((2R,3S,4R,5R)-5-(3-Carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl hydrogen phosphate (cas: 1094-61-7Application In Synthesis of ((2R,3S,4R,5R)-5-(3-Carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl hydrogen phosphate).
((2R,3S,4R,5R)-5-(3-Carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl hydrogen phosphate (cas: 1094-61-7) belongs to amides. Amides are pervasive in nature and technology. Proteins and important plastics like Nylons, Aramid, Twaron, and Kevlar are polymers whose units are connected by amide groups (polyamides); these linkages are easily formed, confer structural rigidity, and resist hydrolysis. Amides are stable compounds. The lower-melting members (such as acetamide) can be readily purified by fractional distillation. Most amides are solids which have low solubilities in water.Application In Synthesis of ((2R,3S,4R,5R)-5-(3-Carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl hydrogen phosphate
Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics