Month: November 2022

On December 29, 2017, Lunn, Daniel B.; Yun, Young J.; Jorgenson, James W. published an article.Product Details of 27115-50-0 The title of the article was Retention and effective diffusion of model metabolites on porous graphitic carbon. And the article contained the following:

The study of metabolites in biol. samples is of high interest for a wide range of biol. and pharmaceutical applications. Reversed phase liquid chromatog. is a common technique used for the separation of metabolites, but it provides little retention for polar metabolites. An alternative to C18 bonded phases, porous graphitic carbon has the ability to provide significant retention for both non-polar and polar analytes. The goal of this work is to study the retention and effective diffusion properties of porous graphitic carbon, to see if it is suitable for the wide injection bands and long run times associated with long, packed capillary-scale separations The retention of a set of standard metabolites was studied for both stationary phases over a wide range of mobile phase conditions. Porous graphitic carbon benefits from significantly increased retention (often >100 fold) under initial gradient conditions for these metabolites, suggesting much improved ability to focus a wide injection band at the column inlet. The effective diffusion properties of these columns were studied using peak-parking experiments with the standard metabolites under a wide range of retention conditions. Under the high retention conditions, which can be associated with retention after injection loading for gradient separations, Deff/Dm ∼ 0.1 for both the C18-bonded and porous graphitic carbon columns. As C18 bonded particles are widely, and successfully used for long gradient separations without issue of increasing peak width from longitudinal diffusion, this suggests that porous graphitic carbon should be amenable for long runtime gradient separations as well. The experimental process involved the reaction of 2-(4-Methylbenzamido)acetic acid(cas: 27115-50-0).Product Details of 27115-50-0

The Article related to metabolite retention diffusion porous graphitic carbon chromatog, effective diffusion, metabolites, porous graphitic carbon, sample pre-concentration, Biochemical Methods: Chromatographic and other aspects.Product Details of 27115-50-0

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

On October 31, 2012, Alwis, K. Udeni; Blount, Benjamin C.; Britt, April S.; Patel, Dhrusti; Ashley, David L. published an article.Category: amides-buliding-blocks The title of the article was Simultaneous analysis of 28 urinary VOC metabolites using ultra high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (UPLC-ESI/MSMS). And the article contained the following:

Volatile organic compounds (VOCs) are ubiquitous in the environment, originating from many different natural and anthropogenic sources, including tobacco smoke. Long-term exposure to certain VOCs may increase the risk for cancer, birth defects, and neurocognitive impairment. Therefore, VOC exposure is an area of significant public health concern. Urinary VOC metabolites are useful biomarkers for assessing VOC exposure because of non-invasiveness of sampling and longer physiol. half-lives of urinary metabolites compared with VOCs in blood and breath. We developed a method using reversed-phase ultra high performance liquid chromatog. (UPLC) coupled with electrospray ionization tandem mass spectrometry (ESI/MSMS) to simultaneously quantify 28 urinary VOC metabolites as biomarkers of exposure. We describe a method that monitors metabolites of acrolein, acrylamide, acrylonitrile, benzene, 1-bromopropane, 1,3-butadiene, carbon-disulfide, crotonaldehyde, cyanide, N,N-dimethylformamide, ethylbenzene, ethylene oxide, propylene oxide, styrene, tetrachloroethylene, toluene, trichloroethylene, vinyl chloride and xylene. The method is accurate (mean accuracy for spiked matrix ranged from 84 to104%), sensitive (limit of detection ranged from 0.5 to 20 ng mL-1) and precise (the relative standard deviations ranged from 2.5 to 11%). We applied this method to urine samples collected from 1203 non-smokers and 347 smokers and demonstrated that smokers have significantly elevated levels of tobacco-related biomarkers compared to non-smokers. We found significant (p < 0.0001) correlations between serum cotinine and most of the tobacco-related biomarkers measured. These findings confirm that this method can effectively quantify urinary VOC metabolites in a population exposed to volatile organics The experimental process involved the reaction of 2-(4-Methylbenzamido)acetic acid(cas: 27115-50-0).Category: amides-buliding-blocks

The Article related to urine voc metabolite uplc ms tobacco smoke biomarker, Toxicology: Methods (Including Analysis) and other aspects.Category: amides-buliding-blocks

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

On December 31, 2014, Meyer-Monath, Marie; Chatellier, Claudine; Rouget, Florence; Morel, Isabelle; Lestremau, Francois published an article.Reference of 2-(4-Methylbenzamido)acetic acid The title of the article was Development of a multi-residue method in a fetal matrix: analysis of meconium. And the article contained the following:

Meconium is the earliest stool of newborns. It is a complex matrix that reflects the degree of fetal exposure to environmental pollutants. To investigate exposure to xenobiotics, an anal. method was developed to identify and quantify some pesticides and their metabolites and BTEX metabolites in meconium. Samples were prepared by two liquid-solid extractions and purified twice using SPE cartridges, followed by anal. with liquid chromatog. coupled with tandem mass spectrometry. SPE cartridges (polymeric phase with hydrophilic and hydrophobic interactions, ion exchange, mixed mode) were tested and matrix effects were evaluated to determine purification performance. The quantification limits in meconium of this multi-residue method were in the range of 30 ng g-1. The anal. method was applied to “real” meconium samples. Some target analytes were determined in most samples. The experimental process involved the reaction of 2-(4-Methylbenzamido)acetic acid(cas: 27115-50-0).Reference of 2-(4-Methylbenzamido)acetic acid

The Article related to pollutant multiresidue analysis fetus meconium lc ms, Toxicology: Methods (Including Analysis) and other aspects.Reference of 2-(4-Methylbenzamido)acetic acid

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

On July 15, 2013, Gagne, Sebastien published an article.Reference of 2-(4-Methylbenzamido)acetic acid The title of the article was Achieving greater selectivity for the analysis of o-, m-, p-methylhippuric acids in workers’ urine by ultra performance liquid chromatography coupled with tandem mass spectrometry. And the article contained the following:

A selective method analyzing sep. o-, m- and p-methylhippuric acid isomers in workers’ urine samples has been developed using ultra performance liquid chromatog. coupled with tandem mass spectrometry. Chromatog. separation has been optimized to resolve the three isomers at baseline. Combined with this optimal separation, the mass spectrometer allowed rapid switching from MRM scan to full scan and product ion scan within the chromatog. peak. This feature allowed the retention of analyte chem. structure information for the three methylhippuric acid isomers in parallel with the simultaneous acquisition of quant. data. Such an approach is unequaled for the reliability of the data generated and it can be applied to each isomer sep. The method was adjusted to a dynamic range between 0.2 mM and 8.12 mM for o-methylhippuric acid and p-methylhippuric acid, and between 0.41 mM and 16.23 mM for m-methylhippuric acid to cover the biol. exposure index. A negligible matrix effect was observed with the conditions used. Also, intra-day and inter-day precisions were both <6% for all the concentration levels tested and the accuracy was evaluated at 97%. The inclusion of simultaneous full scan acquisitions did not prevent the robustness of the quant. data. The method applied to the determination of inter-laboratory proficient test samples led to results in the tolerated range. Moreover, urine samples from workers were robustly quantified and the MHA levels were all below the biol. exposure index reference value. The experimental process involved the reaction of 2-(4-Methylbenzamido)acetic acid(cas: 27115-50-0).Reference of 2-(4-Methylbenzamido)acetic acid

The Article related to xylene exposure methylhippurate urine analysis worker, Toxicology: Methods (Including Analysis) and other aspects.Reference of 2-(4-Methylbenzamido)acetic acid

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Yamamoto, Priscila A.; de Moraes, Natalia V.; Silva, Vinicius P.; Lepera, Jose S. published an article in 2016, the title of the article was Simultaneous analysis of biological indicators of exposure to ethylbenzene, styrene, toluene and xylene solvents in urine by HPLC-UV.Recommanded Product: 2-(4-Methylbenzamido)acetic acid And the article contains the following content:

A method for simultaneous anal. of phenylglyoxylic (PGA), mandelic (MA), hippuric (HA), ortho-, meta- and para-methylhippuric (o-, m- and p-MHA) acids in urine, by high performance liquid chromatog. with UV detection was developed and validated. The substances are biotransformation products used as biol. indicators to assess the exposure of workers, resp., to ethylbenzene, styrene, toluene and xylene. The urinary metabolites were separated using a C18 column and a mixture of 5 mM phosphate buffer pH 2.6:acetonitrile (92:8, volume/volume) as mobile phase. Urine samples were extracted with dichloromethane:ethyl acetate (70:30, volume/volume). The method used phenacetin as internal standard and was linear in the interval of 100-500 μg mL-1 for PGA and MA and 150-700 μg mL-1 for HA and MHA. The detection limits in μg mL-1, were 19.8 for PGA, 1.7 for MA, 4.1 for HA, 3.9 for o-MHA, 3.3 for m-MHA and 7.3 for p-MHA. Intra- and inter-assay precisions (as relative standard deviation) were all less than 15% and accuracy (as relative standard error) did not exceed 12.3%. The recovery was higher than 65% for all metabolites. The developed method will be applied to biol. evaluation of workers exposure to these solvents. The experimental process involved the reaction of 2-(4-Methylbenzamido)acetic acid(cas: 27115-50-0).Recommanded Product: 2-(4-Methylbenzamido)acetic acid

The Article related to volatile organic compound exposure urine analysis hplc uv, Toxicology: Methods (Including Analysis) and other aspects.Recommanded Product: 2-(4-Methylbenzamido)acetic acid

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

On April 30, 2015, Schantz, Michele M.; Benner, Bruce A. Jr.; Heckert, N. Alan; Sander, Lane C.; Sharpless, Katherine E.; Vander Pol, Stacy S.; Vasquez, Y.; Villegas, M.; Wise, Stephen A.; Alwis, K. Udeni; Blount, Benjamin C.; Calafat, Antonia M.; Li, Zheng; Silva, Manori J.; Ye, Xiaoyun; Gaudreau, Eric; Patterson, Donald G. Jr.; Sjodin, Andreas published an article.Formula: C10H11NO3 The title of the article was Development of urine standard reference materials for metabolites of organic chemicals including polycyclic aromatic hydrocarbons, phthalates, phenols, parabens, and volatile organic compounds. And the article contained the following:

Two new Standard Reference Materials (SRMs), SRM 3672 Organic Contaminants in Smokers’ Urine (Frozen) and SRM 3673 Organic Contaminants in Non-Smokers’ Urine (Frozen), have been developed in support of studies for assessment of human exposure to select organic environmental contaminants. Collaborations among three organizations resulted in certified values for 11 hydroxylated polycyclic aromatic hydrocarbons (OH-PAHs) and reference values for 11 phthalate metabolites, 8 environmental phenols and parabens, and 24 volatile organic compound (VOC) metabolites. Reference values are also available for creatinine and the free forms of caffeine, theobromine, ibuprofen, nicotine, cotinine, and 3-hydroxycotinine. These are the first urine Certified Reference Materials characterized for metabolites of organic environmental contaminants. Noteworthy, the mass fractions of the environmental organic contaminants in the two SRMs are within the ranges reported in population survey studies such as the National Health and Nutrition Examination Survey (NHANES) and the Canadian Health Measures Survey (CHMS). These SRMs will be useful as quality control samples for ensuring compatibility of results among population survey studies and will fill a void to assess the accuracy of anal. methods used in studies monitoring human exposure to these organic environmental contaminants. The experimental process involved the reaction of 2-(4-Methylbenzamido)acetic acid(cas: 27115-50-0).Formula: C10H11NO3

The Article related to urine metabolite pah phthalate phenol paraben volatile standard reference, Toxicology: Methods (Including Analysis) and other aspects.Formula: C10H11NO3

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

On July 31, 2019, Cook, Daniel W.; Oram, Kelson G.; Rutan, Sarah C.; Stoll, Dwight R. published an article.Recommanded Product: 27115-50-0 The title of the article was Rational design of mixtures for chromatographic peak tracking applications via multivariate selectivity. And the article contained the following:

Chromatog. characterization and parameterization studies targeting many solutes require the judicious choice of operating conditions to minimize anal. time without compromising the accuracy of the results. To minimize anal. time, solutes are often grouped into a small number of mixtures; however, this increases the risk of peak overlap. While multivariate curve resolution methods are often able to resolve analyte signals based on their spectral qualities, these methods require that the chromatog. overlapped compounds have dissimilar spectra. In this work, a strategy for grouping compounds into sample mixtures containing solutes with distinct spectral and, optionally, with distinct chromatog. properties, in order to ensure successful solute resolution either chromatog. or with curve resolution methods is proposed. We name this strategy rational design of mixtures (RDM). RDM utilizes multivariate selectivity as a metric for making decisions regarding group membership (i.e., whether to add a particular solute to a particular sample). A group of 97 solutes was used to demonstrate this strategy. Utilizing both estimated chromatog. properties and measured spectra to group these 97 analytes, only 12 groups were required to avoid a situation where two or more solutes in the same group could not be resolved either chromatog. (i.e., they have significantly different retention times) or spectrally (i.e., spectra are different enough to enable resolution by curve resolution methods). When only spectral properties were utilized (i.e., the chromatog. properties are unknown ahead of time) the number of groups required to avoid unresolvable overlaps increased to 20. The grouping strategy developed here will improve the time and instrument efficiency of studies that aim to obtain retention data for solutes as a function of operating conditions, whether for method development or determination of the chromatog. parameters of solutes of interest (e.g., kw). The experimental process involved the reaction of 2-(4-Methylbenzamido)acetic acid(cas: 27115-50-0).Recommanded Product: 27115-50-0

The Article related to analyte adsorption chromatog spectra, multivariate curve resolution, net analyte signal, rational design of mixtures, Organic Analytical Chemistry: Detections and other aspects.Recommanded Product: 27115-50-0

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

On November 30, 2021, Toffa, Joelle; Loko, Yeyinou Laura Estelle; Djedatin, Gustave; Gbemavo, Charlemagne D. S. J.; Orobiyi, Azize; Tchakpa, Cyrille; Ewedje, Eben-Ezer; Sabot, Francois published an article.HPLC of Formula: 79-07-2 The title of the article was Rice pests in the Republic of Benin : farmers’ perceptions, knowledge and management practices. And the article contained the following:

Rice (Oryza spp) is one of the most consumed cereals in the Republic of Benin. However, rice production is threatened by various pests, which lead to important yield losses. For the development of integrated management strategies responding to the farmers’ realities, it is important to document their perceptions, knowledge and management of rice pests. Surveys involving 418 rice farmers to 21 ethnic groups through 39 villages were performed using rural appraisal tools. Farmers perceived birds, specifically weavers as the most important rice pests. The surveyed farmers also identified the variegated grasshopper, Zonocerus variegatus L. and rice brown leaf spots [Curvularia lunata (Wakker) Boedijn] as the main pests in the northern region, the pink stem borer, Sesamia calamistis Hampson and rice blast (Magnaporthe grisea (Hebert) Barr) in the southern region, and Z. variegatus and rice yellow mottle virus (genus Sobemovirus) in central Benin. The most important rice storage constraint was rodent attacks and the surveyed farmers proposed 13 key solutions to minimize constraints related to rice storage. Among various pest control methods recorded, farmers used mainly synthetic chem. pesticides. However, the Beninese National Pesticide Management Committee (CNGP) does not recommend most of pesticides used by farmers for rice protection (prohibited pesticides or intended for the protection of other crops). Farming experience, family size, region and number of observed pests have significantly influenced farmers’ decision to use pesticides. The rice pests perceived by farmers as important vary significantly across regions. These results suggest that integrated pest management programs which target rice pests accounting for these regional differences will be more effective. The identified variables that influence the use of pesticides must be taken in account in the development of strategies which will encourage farmers to use ecofriendly pest management. The experimental process involved the reaction of 2-Chloroacetamide(cas: 79-07-2).HPLC of Formula: 79-07-2

The Article related to farmer rice pest management practice, birds, diseases, insect pests, pesticides, rodents, traditional control, weeds, Agrochemical Bioregulators: Invertebrate and other aspects.HPLC of Formula: 79-07-2

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Toda, Yasunori; Tanaka, Katsumi; Matsuda, Riki; Suga, Hiroyuki published an article in 2019, the title of the article was Visible-light-triggered Catalytic Halohydrin Synthesis from Epoxides and Trichloroacetonitrile by Copper and Iron Salts.Related Products of 5455-98-1 And the article contains the following content:

Preparation of vicinal halohydrins by the ring-opening reaction of epoxides under visible-light conditions using copper or iron chlorides catalyst was described. The use of trichloroacetonitrile as a chloride source enabled catalytic HCl generation under the mild conditions. This method can also be applied to the aziridine ring-opening reaction. The experimental process involved the reaction of 2-(Oxiran-2-ylmethyl)isoindoline-1,3-dione(cas: 5455-98-1).Related Products of 5455-98-1

The Article related to epoxide trichloroacetonitrile metal chloride catalyst regioselective photochem ring opening, chlorohydrin preparation, Aliphatic Compounds: Alcohols and Thiols and other aspects.Related Products of 5455-98-1

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

On December 15, 2017, Gonzalez, Johannes L.; Pell, Albert; Lopez-Mesas, Montserrat; Valiente, Manuel published an article.HPLC of Formula: 27115-50-0 The title of the article was Simultaneous determination of BTEX and their metabolites using solid-phase microextraction followed by HPLC or GC/MS: An application in teeth as environmental biomarkers. And the article contained the following:

Applications of benzene, toluene, ethylbenzene, and o-, m-, and p-xylenes (BTEX) release them into the environment exposing living organism. These endocrine disruptors are toxic, highly volatile and easily absorbed by the lungs and can cause adverse consequences for the human health as neurol. diseases and cancer. A method for the anal. of BTEX and its metabolites (phenols and aromatic acids) in teeth is presented. The method consists in a one-step simple extraction procedure from spiked tooth using NaOH solution followed by SPME-HPLC or HS-SPME-GC/MS determination Optimization of both, spiking procedure and extraction step of these analytes from tooth, was carried out. Two fibers CAR/PDMS for BTEX and PA for BTEX metabolites were used for the SPME and variables were optimized for analytes at 30° using spiked solutions The optimized adsorption times were 30, 75 and 30 min and desorption times were 10, 40 and 30 min for BTEX, phenols and aromatic acids, resp. Linearity for SPME-HPLC method was established using spiked solutions with both, BTEX and metabolites, at 2.5, 5.0, 10.0, 25.0 μg/mL. The obtained results indicated a good linearity (R2 above 0.994) for all analytes. Triplicate analyses were performed with RSD lower than 15%. LODs were in the range 0.2-33.3 ng/mL for SPME-HPLC and 0.06-0.09 pg/mL for HS-SPME-GC/MS methods in spiking solutions Once the method was optimized, bovine teeth were used as biol. matrix model for the tuning of spiking and extraction steps. Optimal adsorption and desorption times were 4 h for both procedures. Micrograms per tooth gram of BTEX and phenols were quantified in ten human teeth and aromatic acids were not identified. The developed method for BTEX and metabolites analyses using SPME-HPLC or HS-SPME-GC/MS shows good precision, linearity and sensitivity. The method was successfully applied in human teeth as environmental biomarker of BTEX and metabolites. The experimental process involved the reaction of 2-(4-Methylbenzamido)acetic acid(cas: 27115-50-0).HPLC of Formula: 27115-50-0

The Article related to btex metabolite biomarker determination tooth hplc gc ms, btex, btex metabolites, hplc, gc–ms, human teeth samples, spme, Toxicology: Methods (Including Analysis) and other aspects.HPLC of Formula: 27115-50-0

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics