Month: November 2022

Bruun, L. published the artcileA quantitative enzyme-linked immunoassay for the detection of 2,6-dichlorobenzamide (BAM), a degradation product of the herbicide dichlobenil, Name: 2,4-Dichlorobenzamide, the publication is Journal of Immunological Methods (2000), 240(1-2), 133-142, database is CAplus and MEDLINE.

2,6-Dichlorobenzamide (BAM) is the dominant degradation product in soil of the widely used herbicide dichlobenil. To detect BAM in water, a highly sensitive and specific ELISA was developed. As an alternative to conventional coating of ELISA plates, the assay is based on direct covalent immobilization. A surface was achieved which requires a short time for the immobilization of ligand, is stable under dry storage, and which permits assays with a low CV. The performance of the assay was demonstrated by an inter-well CV that was generally less than 6%, a detection limit (DL₁₅) of 0.02 μg/l and an IC₅₀ of 0.19 μg/l. Cross-reactivity was measured against nine analytes with structural homol. to BAM. The highest degree of cross-reactivity (10.8%) was seen with 2,6-dichlorothiobenzamide (Chlorthiamid). Considering an EU-limit of 0.1 μg/l as the permissible maximum for the presence of pesticides in drinking water, this ELISA-procedure is suitable for large-scale screening of water samples suspected of being contaminated with BAM.

Journal of Immunological Methods published new progress about 2447-79-2. 2447-79-2 belongs to amides-buliding-blocks, auxiliary class Chloride,Amine,Benzene,Amide, name is 2,4-Dichlorobenzamide, and the molecular formula is C7H5Cl2NO, Name: 2,4-Dichlorobenzamide.

Referemce:
https://en.wikipedia.org/wiki/Amide,
Amide – an overview | ScienceDirect Topics

Socher, Elke published the artcileDual fluorophore PNA FIT-probes – extremely responsive and bright hybridization probes for the sensitive detection of DNA and RNA, Synthetic Route of 186046-83-3, the publication is Organic & Biomolecular Chemistry (2012), 10(36), 7363-7371, database is CAplus and MEDLINE.

Fluorescently labeled oligonucleotides are commonly employed as probes to detect specific DNA or RNA sequences in homogeneous solution Useful probes should experience strong increases in fluorescent emission upon hybridization with the target. Dual labeled peptide nucleic acid probes were developed which signal the presence of complementary DNA or RNA by up to 450-fold enhancements of fluorescence intensity. This enabled the very sensitive detection of a DNA target (40 pM LOD), which was detectable at less than 0.1% of the beacon concentration In contrast to existing DNA-based mol. beacons, this PNA-based method does not require a stem sequence to enforce dye-dye communication. Rather, the method relies on the energy transfer between a “smart” thiazole orange (TO) nucleotide, which requires formation of the probe-target complex in order to become fluorescent, and terminally appended acceptor dyes. To improve upon fluorescence responsiveness the energy pathways were dissected. Hydrophobic, spectrally mismatched dye combinations allowed significant (99.97%) decreases of background emission in the absence of a target. By contrast, spectral overlap between TO donor emission and acceptor excitation enabled extremely bright FRET signals. This and the large apparent Stokes shift (82 nm) suggests potential applications in the detection of specific RNA targets in biogenic matrixes without the need of sample pre-processing prior to detection.

Organic & Biomolecular Chemistry published new progress about 186046-83-3. 186046-83-3 belongs to amides-buliding-blocks, auxiliary class Purine,Carboxylic acid,Amine,Benzene,Amide,Others,PNA,, name is 2-(N-(2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)ethyl)-2-(2-(((benzhydryloxy)carbonyl)amino)-6-oxo-5H-purin-9(6H)-yl)acetamido)acetic acid, and the molecular formula is C12H14IN, Synthetic Route of 186046-83-3.

Referemce:
https://en.wikipedia.org/wiki/Amide,
Amide – an overview | ScienceDirect Topics

Kummer, Susann published the artcileFluorescence imaging of influenza H1N1 mRNA in living infected cells using single-chromophore FIT-PNA, COA of Formula: C40H35N7O8, the publication is Angewandte Chemie, International Edition (2011), 50(8), 1931-1934, S1931/1-S1931/22, database is CAplus and MEDLINE.

The FIT-PNA probes used in this work contain a single thiazole orange (TO) intercalator serving as artificial fluorescent nucleobase. These probes respond to changes of the local structure in the vicinity of the dye rather than to the more global changes of conformation that confer fluorescence signaling by dual-labeled mol. beacons. Herein we demonstrate the advantageous properties of FIT-PNA probes in the imaging of mRNA from an influenza virus strain belonging to the same subtype as the recently emerged swine virus (A/Mexico/1/2009, H1N1).

Angewandte Chemie, International Edition published new progress about 186046-83-3. 186046-83-3 belongs to amides-buliding-blocks, auxiliary class Purine,Carboxylic acid,Amine,Benzene,Amide,Others,PNA,, name is 2-(N-(2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)ethyl)-2-(2-(((benzhydryloxy)carbonyl)amino)-6-oxo-5H-purin-9(6H)-yl)acetamido)acetic acid, and the molecular formula is C40H35N7O8, COA of Formula: C40H35N7O8.

Referemce:
https://en.wikipedia.org/wiki/Amide,
Amide – an overview | ScienceDirect Topics

Kummer, Susann published the artcilePNA FIT-Probes for the Dual Color Imaging of Two Viral mRNA Targets in Influenza H1N1 Infected Live Cells, Safety of 2-(N-(2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)ethyl)-2-(2-(((benzhydryloxy)carbonyl)amino)-6-oxo-5H-purin-9(6H)-yl)acetamido)acetic acid, the publication is Bioconjugate Chemistry (2012), 23(10), 2051-2060, database is CAplus and MEDLINE.

Fluorogenic hybridization probes that allow RNA imaging provide information as to how the synthesis and transport of particular RNA mols. is orchestrated in living cells. The authors explored the peptide nucleic acid (PNA)-based FIT-probes in the simultaneous imaging of two different viral mRNA mols. expressed during the replication cycle of the H1N1 influenza A virus. PNA FIT-probes are non-nucleotidic, nonstructured probes and contain a single asym. cyanine dye which serves as a fluorescent base surrogate. The fluorochrome acts as a local intercalator probe and reports hybridization of target DNA/RNA by enhancement of fluorescence. Though multiplexed hybridization probes are expected to facilitate the anal. of RNA expression, there are no previous reports on the dual color imaging of two different viral mRNA targets. The authors developed a set of two differently colored PNA FIT-probes that allow the spectrally resolved imaging of mRNA coding for neuraminidase (NA) and matrix protein 1 (M1); proteins which execute distinct functions during the replication of the influenza A virus. The probes were characterized by a wide range of applicable hybridization temperatures The same probe sequence enabled live-cell RNA imaging (at 37°) as well as real-time PCR measurements (at 60° annealing temperature). This facilitated a comprehensive anal. of RNA expression by quant. (qPCR) and qual. (imaging) means. Confocal laser scanning microscopy showed that the viral-RNA specific PNA FIT-probes neither stained noninfected cells nor cells infected by a control virus. The joint use of differently colored PNA FIT-probes in this feasibility study revealed significant differences in the expression pattern of influenza H1N1 mRNAs coding for NA or M1. These experiments provide evidence for the usefulness of PNA FIT-probes in studies on the temporal and spatial progression of mRNA synthesis in living cells for two mRNA species.

Bioconjugate Chemistry published new progress about 186046-83-3. 186046-83-3 belongs to amides-buliding-blocks, auxiliary class Purine,Carboxylic acid,Amine,Benzene,Amide,Others,PNA,, name is 2-(N-(2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)ethyl)-2-(2-(((benzhydryloxy)carbonyl)amino)-6-oxo-5H-purin-9(6H)-yl)acetamido)acetic acid, and the molecular formula is C40H35N7O8, Safety of 2-(N-(2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)ethyl)-2-(2-(((benzhydryloxy)carbonyl)amino)-6-oxo-5H-purin-9(6H)-yl)acetamido)acetic acid.

Referemce:
https://en.wikipedia.org/wiki/Amide,
Amide – an overview | ScienceDirect Topics

Kramarczyk, Daniel published the artcileInhibition of celecoxib crystallization by mesoporous silica – Molecular dynamics studies leading to the discovery of the stabilization origin, Synthetic Route of 169590-42-5, the publication is European Journal of Pharmaceutical Sciences (2022), 106132, database is CAplus and MEDLINE.

In this article, the effect of mesoporous silica (MS) on the phys. stability and mol. dynamics of the amorphous form of Celecoxib (CEL) is investigated. It has been proven that the recrystallization process of CEL slows down with increasing the MS content. Beside the elongation of stabilization time with the increase silica content leads to an increase in the amorphous drug fraction remaining after the finished crystallization The conducted analyses show that the observed inhibition of CEL’s recrystallization is associated with the formation of a monomol. drug layer on the silica’s surface. The performed non-isothermal dielec. studies of CEL + MS systems having both fully and partially amorphous CEL shows that the biggest impact of the drug’s the temperature dependences of structural relaxation time τα(T) has a crystalline fraction of the API. Silica, even in high concentration, does not modify the temperature dependence of structural relaxation of CEL.

European Journal of Pharmaceutical Sciences published new progress about 169590-42-5. 169590-42-5 belongs to amides-buliding-blocks, auxiliary class Sulfamide,Immunology/Inflammation,COX, name is 4-(5-(p-Tolyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl)benzenesulfonamide, and the molecular formula is C17H14F3N3O2S, Synthetic Route of 169590-42-5.

Referemce:
https://en.wikipedia.org/wiki/Amide,
Amide – an overview | ScienceDirect Topics

Schroth, Werner published the artcileDehydration of ureas with dichlorocarbene in the phase transfer method, Computed Properties of 2451-91-4, the publication is Zeitschrift fuer Chemie (1981), 21(1), 25-7, database is CAplus.

Mono- and disubstituted ureas RR¹NCONH₂ [R = alkyl, R¹ = H, alkyl, or Ph; R = R¹ = PhCH₂; or RR¹ = (CH₂)₄, (CH₂)₅, oxydiethylene, or (phenylimino) diethylene] were dehydrated by treatment with :CCl₂ in the presence of a phase-transfer catalyst, (PhCH₂)Et₃NCl, to give 20-85% RR¹NCN. (CH₂)₄NCSNH₂ was obtained in 45% yield by the addition of H₂S to (CH₂)₄NCN in the presence of an equimolar amount of dry Et₃N.

Zeitschrift fuer Chemie published new progress about 2451-91-4. 2451-91-4 belongs to amides-buliding-blocks, auxiliary class Nitrile,Amine,Benzene, name is N,N-Dibenzylcyanamide, and the molecular formula is C15H14N2, Computed Properties of 2451-91-4.

Referemce:
https://en.wikipedia.org/wiki/Amide,
Amide – an overview | ScienceDirect Topics

Schroth, W. published the artcileThe dehydration of ureas by two-phase dichlorocarbene reaction, a synthetic access to substituted cyanamides, Category: amides-buliding-blocks, the publication is Journal fuer Praktische Chemie (Leipzig) (1983), 325(5), 787-802, database is CAplus.

A wide variety of N,N-disubstituted ureas were dehydrated in the CHCl₃/NaOH catalytic 2-phase system under mild conditions. The sequence of urea transamidation and dehydration offers a profitable approach to aprotic cyanamides. Among various phase-transfer catalysts tertiary amines prove to be the most efficient. Tertiary amines may also be used in the transformation of carboxamides and thioamides to the corresponding nitriles. The application of the same technique is less suitable in the case of N-monosubstituted ureas, N,N‘-disubstituted ureas, and N-(dialkylaminomethylene)ureas, since subsequent reactions of the cyanamides predominate. The dehydration mechanism is elucidated in terms of HOMO-perturbation theory.

Journal fuer Praktische Chemie (Leipzig) published new progress about 2451-91-4. 2451-91-4 belongs to amides-buliding-blocks, auxiliary class Nitrile,Amine,Benzene, name is N,N-Dibenzylcyanamide, and the molecular formula is C15H14N2, Category: amides-buliding-blocks.

Referemce:
https://en.wikipedia.org/wiki/Amide,
Amide – an overview | ScienceDirect Topics

McCall, John M. published the artcileCyanoimine chemistry: new routes to pyrimidinones and (carbonylamino)iminopropanamides, Recommanded Product: 2-Cyano-N-ethylacetamide, the publication is Journal of Organic Chemistry (1979), 44(9), 1562-3, database is CAplus.

RNHCOCH₂C(NR¹₂):NCN (I; R = Et, Bu; NR¹₂ = NEt₂, morpholino, piperidino) are versatile precursors of specific N-alkylated pyrimidinones. I are readily converted via KOCMe₃ to II. I can also be hydrolyzed to RNHCOCH₂C(NR¹₂):NCONH₂.

Journal of Organic Chemistry published new progress about 15029-36-4. 15029-36-4 belongs to amides-buliding-blocks, auxiliary class Nitrile,Amine,Aliphatic hydrocarbon chain,Amide, name is 2-Cyano-N-ethylacetamide, and the molecular formula is C5H8N2O, Recommanded Product: 2-Cyano-N-ethylacetamide.

Referemce:
https://en.wikipedia.org/wiki/Amide,
Amide – an overview | ScienceDirect Topics

Fuhrimann, Samuel published the artcileQualitative assessment of 27 current-use pesticides in air at 20 sampling sites across Africa, Application In Synthesis of 79-07-2, the publication is Chemosphere (2020), 127333, database is CAplus and MEDLINE.

Increasing use of current-use pesticides (CUPs) in Africa raises environmental and public health concerns. But there is a large uncertainty about their occurrence and the composition of pesticide mixtures on this continent. This paper investigates the presence of 27 CUPs in air across 20 sampling sites in Africa. 166 passive air samples, consisting of polyurethane foam (PUF), were collected in 12 African countries between 2010 and 2018. Samples were extracted with methanol and analyzed via high-performance liquid chromatog. coupled with tandem mass spectrometry. The detection frequencies of CUPs per site were compared to land use patterns and sampling years, while their similarities were assessed using hierarchical cluster anal. Atrazine and chlorpyrifos were detected in 19 out of 20 sampling sites. Carbaryl, metazachlor, simazine, tebuconazole and terbuthylazine had the highest detection frequencies at sampling sites dominated by croplands. Across all the sampling years, 16 CUPs were present. Seven CUPs were newly detected from 2016 onwards (azinfos-Me, dimetachlor, chlorsulfuron, chlortoluron, isoproturon, prochloraz and pyrazon), while metamitron was only present before 2012. Sites within a radius of about 200 km showed similarities in detected CUP mixtures across all samples. Our results show the presence of CUP mixtures across multiple agricultural and urban locations in Africa which requires further investigation of related environmental and human health risks.

Chemosphere published new progress about 79-07-2. 79-07-2 belongs to amides-buliding-blocks, auxiliary class Chloride,Amine,Aliphatic hydrocarbon chain,Amide,Inhibitor, name is 2-Chloroacetamide, and the molecular formula is C2H4ClNO, Application In Synthesis of 79-07-2.

Referemce:
https://en.wikipedia.org/wiki/Amide,
Amide – an overview | ScienceDirect Topics

Katritzky, Alan R. published the artcileSynthesis of mono- and N,N-disubstituted thioureas and N-acylthioureas, SDS of cas: 14294-10-1, the publication is Synthesis (2004), 1799-1805, database is CAplus.

1-Benzotriazole-1-carbothioamide, prepared from 1-cyanobenzotriazole and hydrogen sulfide, reacts with amines to give thioureas R¹NR²CSNH₂ (R¹ = MeOC₆H₄, Bn, pyrrolidinyl, Ph, PhNH; R² = H, Bn). Reactions of (benzotriazol-1-yl)carboximidamides and acyl- or arylaminocarbonyl(benzotriazol-1-yl)carboximidamides with hydrogen sulfide give the corresponding thioureas, N-acylthioureas, or N-carbamoylthioureas.

Synthesis published new progress about 14294-10-1. 14294-10-1 belongs to amides-buliding-blocks, auxiliary class Morpholine,Thiourea,Amine,Amide, name is Morpholine-4-carbothioamide, and the molecular formula is C5H10N2OS, SDS of cas: 14294-10-1.

Referemce:
https://en.wikipedia.org/wiki/Amide,
Amide – an overview | ScienceDirect Topics