Anabuki, Tomoaki published the artcileNovel biotin linker with alkyne and amino groups for chemical labelling of a target protein of a bioactive small molecule, SDS of cas: 2418-95-3, the publication is Bioorganic & Medicinal Chemistry Letters (2018), 28(4), 783-786, database is CAplus and MEDLINE.
We synthesized a novel linker (I) with biotin, alkyne and amino groups for the identification of target proteins using a small mol. that contains an azide group (azide probe). The alkyne in the linker bound the azide probe via an azide-alkyne Huisgen cycloaddition A protein cross-linker effectively bound the conjugate of the linker and an azide probe with a target protein. The covalently bound complex was detected by western blotting. Linker I was applied to a model system using an abscisic acid receptor, RCAR/PYR/PYL (PYL). Cross-linked complexes of linker I, the azide probes and the target proteins were successfully visualized by western blotting. This method of target protein identification was more effective than a previously developed method that uses a second linker with biotin, alkyne, and benzophenone (linker II) that acts to photo-crosslink target proteins. The system developed in this study is a method for identifying the target proteins of small bioactive mols. and is different from photo-affinity labeling.
Bioorganic & Medicinal Chemistry Letters published new progress about 2418-95-3. 2418-95-3 belongs to amides-buliding-blocks, auxiliary class Chiral,Carboxylic acid,Amine,Aliphatic hydrocarbon chain,Ester,Amino acide derivatives, name is H-Lys(Boc)-OH, and the molecular formula is C11H22N2O4, SDS of cas: 2418-95-3.
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