Ethyl acetate fraction of Fagara zanthoxyloides root-bark possess antidiabetic property against alloxan-induced diabetes and its complications in Wistar rat model was written by Amah, Christian Chijioke;Joshua, Parker Elijah;Ekpo, Daniel Emmanuel;Okoro, Jacob Ikechukwu;Asomadu, Rita Onyekachukwu;Obelenwa, Ursula Chidimma;Odiba, Arome Solomon. And the article was included in Journal of Ethnopharmacology in 2022.Reference of 10238-21-8 This article mentions the following:
Fagara zanthoxyloides Lam., an African traditional medicinal plant, is used for treatment of malaria and diabetes. To investigate the antidiabetic property of Et acetate fraction of F. zanthoxyloides root-bark (EAFFZRB) on alloxan-induced diabetic rats. Extraction, isolation, preliminary phytochem. anal., and acute toxicity study of ethanol extract and fractions of F. zanthoxyloides root-bark were achieved using stdandard methods. Phyto-constituents in EAFFZRB were identified using HPLC technique. Forty-eight male Wistar rats (140-185 g) were randomized into 6 groups (n = 8). Groups 1 and 2 served as normal and neg. controls, resp. Diabetes was induced in test groups (2-6) using 150 mg/kg body weight (b.w) Alloxan monohydrate. Rats in groups 4-6 received of 200, 400 and 600 mg/kg b.w. EAFFZRB orally, resp., for 21 days. Group 3 rats received 5 mg/kg b.w Glibenclamide. The effect of EAFFZRB on alterations in hematol., biochem., and histol. indexes of study rats were assessed. Extraction of 3500 g ethanol extract yielded 15.71 g EAFFZRB. HPLC fingerprint of EAFFZRB indicated presence of luteolin, rutin, quercetin, apigenin, cinnamic acid and catechin. Diabetes triggered significant (p < 0.05) alterations in b.w., hematol., biochem. and histol. indexes of test rats relative to normal control. Treatment with EAFFZRB (LD50 = 3807.9 mg/kg b.w.) resulted in remarkable improvements in altered b.w. changes, hematol., biochem. and histolol. parameters of diabetic rats. The study demonstrated the antidiabetic potential of EAFFZRB, providing scientific basis for traditional use of the plant in treatment of diabetes and its complications. In the experiment, the researchers used many compounds, for example, 5-Chloro-N-(4-(N-(cyclohexylcarbamoyl)sulfamoyl)phenethyl)-2-methoxybenzamide (cas: 10238-21-8Reference of 10238-21-8).
5-Chloro-N-(4-(N-(cyclohexylcarbamoyl)sulfamoyl)phenethyl)-2-methoxybenzamide (cas: 10238-21-8) belongs to amides. Because of the greater electronegativity of oxygen, the carbonyl (C=O) is a stronger dipole than the N–C dipole. The presence of a C=O dipole and, to a lesser extent a N–C dipole, allows amides to act as H-bond acceptors. Amides can be recrystallised from large quantities of water, ethanol, ethanol/ether, aqueous ethanol, chloroform/toluene, chloroform or acetic acid. The likely impurities are the parent acids or the alkyl esters from which they have been made. The former can be removed by thorough washing with aqueous ammonia followed by recrystallisation, whereas elimination of the latter is by trituration or recrystallisation from an organic solvent.Reference of 10238-21-8
Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics