Quantification and method validation of dicyclohexylurea content in fosaprepitant dimeglumine drug substance by reverse phase HPLC was written by Kishore, M. V.;Sivarao, T.;Srinivas, K. Rama;Satyendranath, Ch. V.;Kumar, Vundavilli Jagadeesh;Kumar, K. S. R. Pavan;Sreenivas, N.. And the article was included in International Journal of Pharmaceutical Sciences and Research in 2020.Computed Properties of C13H24N2O This article mentions the following:
A simple, sensitive, and rapid reverse phase High-Pressure Liquid Chromatog. (HPLC) method was developed and validated for the quantification of residual Dicyclohexylurea in Fosaprepitant dimeglumine drug substance at trace level. The method was optimized on Zorbax Eclipse XDB-C8, 250 mm x 4.6 mm, 5μm with column oven temperature maintaining at 35°C with flow rate at 0.7 mL/min. Mobile Phase was prepared by a mixture of phosphate buffer pH 7.0 ((0.68 g of Potassium dihydrogen orthophosphate in 1000 mL of water) and Acetonitrile in the ratio of 53:47 volume/volume), the injection volume is 20μl, the detector wavelength is 205 nm. The developed, optimized method was further validated in accordance with ICH, and the method is found to be specific, sensitive, accurate, and precise. The Limit of detection (LOD) and Limit of quantification (LOQ) for dicyclohexylurea are 20μg/g and 60μg/g, resp. The method validation exptl. results are discussed in detail in this research paper. In the experiment, the researchers used many compounds, for example, 1,3-Dicyclohexylurea (cas: 2387-23-7Computed Properties of C13H24N2O).
1,3-Dicyclohexylurea (cas: 2387-23-7) belongs to amides. Amides can be viewed as a derivative of a carboxylic acid RC(=O)OH with the hydroxyl group –OH replaced by an amine group −NR′R″; or, equivalently, an acyl (alkanoyl) group RC(=O)− joined to an amine group. As a result of interactions such as these, the water solubility of amides is greater than that of corresponding hydrocarbons. These hydrogen bonds are also have an important role in the secondary structure of proteins.Computed Properties of C13H24N2O
Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics