Simultaneous quantification of three amides by gas chromatography time of flight mass spectrometry in extracts of Piper sarmentosum was written by Hussain, Khalid;Ismail, Zhari;Sadikun, Amirin;Ibrahim, Pazilah. And the article was included in Analytical Chemistry: An Indian Journal in 2009.Product Details of 18836-52-7 The following contents are mentioned in the article:
Piper sarmentosum is well known due to its culinary and medicinal properties and has a great potential of commercialization. Herbal standardization is a tedious task mainly due to un-availability or inadequacy of methods and standards, and its lacking is the single biggest hindrance in the acceptance of herbal products in main stream of pharmaceuticals. Therefore, present study aimed to develop an anal. method using gas chromatog. time of flight mass spectrometry for the simultaneous quantification of three amides, which can be used as pharmacol. active anal. markers to standardize the product made from this plant and others containing these compounds Three amides, pellitorine, sarmentine, and sarmentosine isolated and identified previously from fruit of the plant were used as markers to develop and validate the method. Lowest limit of detection (LOD) of the three amides were found to be 0.10, 0.10 and 0.12 ng/mL, resp., at a signal to noise ratio 3:1, whereas the lowest limit of quantification (LOQ) was taken 1.00, 1.00, and 12.00 ng/mL, resp., at a signal to noise ration 10:1. The method was found to be linear (R2 = 0.9985 to 0.9995) with RSD < 5%. Intraday and inter day accuracy was found to be 97.40-100.00% with precision (RSD <5%). The percentage recoveries were 97-100% with RSD <5%. The method was found sensitive and reproducible, and applied successfully to quantify the amides in ethanol and supercritical CO2 extracts of fruit of Piper sarmentosum. The method is found to be simple, fast and easy to perform, and may be helpful for natural product industry as well as natural product scientists to produce standardized extracts and products from the plant. This study involved multiple reactions and reactants, such as (2E,4E)-N-Isobutyldeca-2,4-dienamide (cas: 18836-52-7Product Details of 18836-52-7).
(2E,4E)-N-Isobutyldeca-2,4-dienamide (cas: 18836-52-7) belongs to amides. Because of the greater electronegativity of oxygen, the carbonyl (C=O) is a stronger dipole than the N–C dipole. The presence of a C=O dipole and, to a lesser extent a N–C dipole, allows amides to act as H-bond acceptors. Amides are stable compounds. The lower-melting members (such as acetamide) can be readily purified by fractional distillation. Most amides are solids which have low solubilities in water.Product Details of 18836-52-7
Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics