Anti-inflammatory and antitumour activity of various extracts and compounds from the fruits of Piper longum L. was written by Guo, Ziyan;Xu, Jie;Xia, Jianhua;Wu, Zi;Lei, Jiachuan;Yu, Jianqing. And the article was included in Journal of Pharmacy and Pharmacology in 2019.COA of Formula: C14H25NO The following contents are mentioned in the article:
Objectives : To explore effective extraction method and to find active constituents, we investigated the biol. activity of three extracts and isolated active compounds from the fruits of Piper longum L. Methods : Three extracts from the fruits were obtained by reflux, ultrasonic and supercritical fluid extraction, resp. Active compounds were isolated by the bioassay-guided method. The anti-inflammatory activity, antiproliferation activity and cytotoxicity were evaluated. The apoptosis was detected by Hoechst 33258 staining assay. The relevant proteins were investigated by Western blot assay. Key findings : The anti-inflammatory activity and cytotoxicity of supercritical fluid extract (SE) were stronger than those of the other two extracts Among all isolated compounds, the anti-inflammatory activity of eight compounds was stronger than that of indomethacin, and compounds 8, 9, 11, 14 and 15 were found to possess anti-inflammatory effect for the first time. Compounds 1, 2, 3 and 14 exhibited significant cytotoxicity against cancer cells. SE and piperine were found to reduce colony formation, inhibit cell migration and promote apoptosis through increasing cleaved PARP and the ratio of Bax/Bcl-2. Conclusions : The anti-inflammatory and antitumor effects of SE were better than those of the other two extracts The compounds responsible for the activity were elucidated. SE and piperine inhibit cell growth through apoptosis. This study involved multiple reactions and reactants, such as (2E,4E)-N-Isobutyldeca-2,4-dienamide (cas: 18836-52-7COA of Formula: C14H25NO).
(2E,4E)-N-Isobutyldeca-2,4-dienamide (cas: 18836-52-7) belongs to amides. Because of the greater electronegativity of oxygen, the carbonyl (C=O) is a stronger dipole than the N–C dipole. The presence of a C=O dipole and, to a lesser extent a N–C dipole, allows amides to act as H-bond acceptors. Amides can be recrystallised from large quantities of water, ethanol, ethanol/ether, aqueous ethanol, chloroform/toluene, chloroform or acetic acid. The likely impurities are the parent acids or the alkyl esters from which they have been made. The former can be removed by thorough washing with aqueous ammonia followed by recrystallisation, whereas elimination of the latter is by trituration or recrystallisation from an organic solvent.COA of Formula: C14H25NO
Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics