Vitamin B12 levels in drug-treated bacterial cells was written by Gajcy, Hanna. And the article was included in Biochemical Pharmacology in 1975.Reference of 7413-34-5 This article mentions the following:
Sulfathiazole Na [144-74-1] (0.05%), amethopterin Na [7413-34-5] (10-4M), and 5-fluorouracil nitrate [57172-36-8] (0.15 mg/ml) partially inhibited the growth of B. megaterium and decreased the vitamin B12 concentrations Similarly, vitamin B12 concentrations and cell growth were decreased at 30 min after treatment with chloramphenicol [56-75-7] (1 μg) or puromycin-2HCl [58-58-2] (2 μg and 4 μg per ml), but then returned to the initial levels. High concentrations of actinomycin D [50-76-0] and chloramphenicol inhibited cell growth but the vitamin B12 content was increased 2-fold. In the experiment, the researchers used many compounds, for example, Sodium (S)-2-(4-(((2,4-diaminopteridin-6-yl)methyl)(methyl)amino)benzamido)pentanedioate (cas: 7413-34-5Reference of 7413-34-5).
Sodium (S)-2-(4-(((2,4-diaminopteridin-6-yl)methyl)(methyl)amino)benzamido)pentanedioate (cas: 7413-34-5) belongs to amides. In primary and secondary amides, the presence of N–H dipoles allows amides to function as H-bond donors as well. Thus amides can participate in hydrogen bonding with water and other protic solvents; the oxygen atom can accept hydrogen bonds from water and the N–H hydrogen atoms can donate H-bonds. As a result of interactions such as these, the water solubility of amides is greater than that of corresponding hydrocarbons. These hydrogen bonds are also have an important role in the secondary structure of proteins.Reference of 7413-34-5
Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics