Chemistry is an experimental science, Recommanded Product: (R)-2-Aminopentanedioic acid, and the best way to enjoy it and learn about it is performing experiments.Introducing a new discovery about 6893-26-1, Name is (R)-2-Aminopentanedioic acid, molecular formula is C5H9NO4, belongs to amides-buliding-blocks compound. In a document, author is Gao, Han.
An NMR Method To Pinpoint Supramolecular Ligand Binding to Basic Residues on Proteins
Targeting protein surfaces involved in protein-protein interactions by using supramolecular chemistry is a rapidly growing field. NMR spectroscopy is the method of choice to map ligand-binding sites with single-residue resolution by amide chemical shift perturbation and line broadening. However, large aromatic ligands affect NMR signals over a greater distance, and the binding site cannot be determined unambiguously by relying on backbone signals only. We herein employed Lys- and Arg-specific H-2(C) N NMR experiments to directly observe the side-chain atoms in close contact with the ligand, for which the largest changes in the NMR signals are expected. The binding of Lys- and Arg-specific supramolecular tweezers and a calixarene to two model proteins was studied. The H-2(C) N spectra track the terminal CH2 groups of all Lys and Arg residues, revealing significant differences in their binding kinetics and chemical shift perturbation, and can be used to clearly pinpoint the order of ligand binding.
Balanced chemical reaction does not necessarily reveal either the individual elementary reactions by which a reaction occurs or its rate law. In my other articles, you can also check out more blogs about 6893-26-1. Recommanded Product: (R)-2-Aminopentanedioic acid.