Author: Jessica.F

Kitadokoro, Kengo; Tanaka, Mutsumi; Hikima, Takaaki; Okuno, Yukiko; Yamamoto, Masaki; Kamitani, Shigeki published the artcile< Crystal structure of pathogenic Staphylococcus aureus lipase complex with the anti-obesity drug orlistat.>, Product Details of C29H53NO5, the main research area is .

Staphylococcus aureus lipase (SAL), a triacylglycerol esterase, is an important virulence factor and may be a therapeutic target for infectious diseases. Herein, we determined the 3D structure of native SAL, the mutated S116A inactive form, and the inhibitor complex using the anti-obesity drug orlistat to aid in drug development. The determined crystal structures showed a typical α/β hydrolase motif with a dimeric form. Fatty acids bound near the active site in native SAL and inactive S116A mutant structures. We found that orlistat potently inhibits SAL activity, and it covalently bound to the catalytic Ser116 residue. This is the first report detailing orlistat-lipase binding. It provides structure-based information on the production of potent anti-SAL drugs and lipase inhibitors. These results also indicated that orlistat can be repositioned to treat bacterial diseases.

Scientific reports published new progress about 96829-58-2. 96829-58-2 belongs to class amides-buliding-blocks, and the molecular formula is C29H53NO5, Product Details of C29H53NO5.

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Hopkins, Megan D.; Ozmer, Garett L.; Witt, Ryan C.; Brandeburg, Zachary C.; Rogers, David A.; Keating, Claire E.; Petcoff, Presley L.; Sheaff, Robert J.; Lamar, Angus A. published the artcile< PhI(OAc)2 and iodine-mediated synthesis of N-alkyl sulfonamides derived from polycyclic aromatic hydrocarbon scaffolds and determination of their antibacterial and cytotoxic activities>, COA of Formula: C6H6ClNO2S, the main research area is alkyl sulfonamide preparation antibacterial antitumor physicochem human.

The development of new approaches toward chemo- and regioselective functionalization of polycyclic aromatic hydrocarbon (PAH) scaffolds will provide opportunities for the synthesis of novel biol. active small mols. that exploit the high degree of lipophilicity imparted by the PAH unit. Herein, new synthetic method for C-X bond substitution that is speculated to operate via a N-centered radical (NCR) mechanism according to exptl. observations was reported. A series of PAH sulfonamides have been synthesized and their biol. activity has been evaluated against Gram-neg. and Gram-pos. bacterial strains (using a BacTiter-Glo assay) along with a series of mammalian cell lines (using CellTiter-Blue and CellTiter-Glo assays). The viability assays have resulted in the discovery of a number of bactericidal compounds that exhibit potency similar to other well-known antibacterials such as kanamycin and tetracycline, along with the discovery of a luciferase inhibitor. Addnl., the physicochem. and drug-likeness properties of the compounds were determined exptl. and using in silico approaches and the results are presented and discussed within.

Organic & Biomolecular Chemistry published new progress about Antibacterial agents. 6961-82-6 belongs to class amides-buliding-blocks, and the molecular formula is C6H6ClNO2S, COA of Formula: C6H6ClNO2S.

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Gomaa, Adel A.; El-Sers, Dalia A.; Al-Zokeim, Nahla I.; Gomaa, Mohamed A. published the artcile< Amelioration of experimental metabolic syndrome induced in rats by orlistat and Corchorus olitorius leaf extract; role of adipo/cytokines>, Recommanded Product: (S)-(S)-1-((2S,3S)-3-Hexyl-4-oxooxetan-2-yl)tridecan-2-yl 2-formamido-4-methylpentanoate, the main research area is orlistat Corchorus olitorius leaf extract metabolic syndrome amelioration cytokines; Corchorus olitorius ; cytokines; histopathology; lipid profile; obesity.

To determine the efficacy of Corchorus olitorius (C. olitorius) leaf extract in the prevention of metabolic syndrome induced in rats by high-fat diet (HFD) and compare it with that of orlistat. Phytochem. anal. was performed. Effect of orlistat and C. olitorius extract on lipase activity and acute food intake were investigated. Body weight, biochem. parameters and histopathol. examination were demonstrated. Corchorus olitorius extract inhibited the pancreatic lipase activity, but orlistat was more potent. Cumulative food intake has not changed by the tested agents. In obese rats, C. olitorius or orlistat significantly decreased weight gain and visceral white adipose tissue. They exhibited a significant reduction in serum glucose, total cholesterol, triglycerides, low d. lipoprotein cholesterol, free fatty acids, IL-1β, tumor necrosis factor-α (TNF-α), insulin and leptin levels of obese rat groups while high d. lipoprotein cholesterol and adiponectin levels were significantly increased by them. Histopathol. examination of the liver revealed that C. olitorius was more effective than orlistat in the alleviating of steatosis and adipocyte hypertrophy shown in obese control rats. Corchorus olitorius is effective as orlistat in preventing obesity, hyperlipidemia, steatosis and insulin resistance. These actions may be mediated by inhibiting of lipase activity, TNF-α, IL-1β and leptin resistance along with increasing of adiponectin.

Journal of Pharmacy and Pharmacology published new progress about Acute toxicity. 96829-58-2 belongs to class amides-buliding-blocks, and the molecular formula is C29H53NO5, Recommanded Product: (S)-(S)-1-((2S,3S)-3-Hexyl-4-oxooxetan-2-yl)tridecan-2-yl 2-formamido-4-methylpentanoate.

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Sharma, Shikha; Ghosh, Sunil K.; Sharma, Joti N. published the artcile< Dialkylmethyl-2-(N,N-diisobutyl)acetamidoammonium iodide as a ruthenium selective ligand from nitric acid medium>, Formula: C10H20ClNO, the main research area is dialkylmethyldiisobutylacetamidoammonium iodide ruthenium ligand nitrate; Alkylammonium acetamides; Iodide ion; Ruthenium; Separation; Solvent extraction.

A new class of quaternary ammonium iodide based ligands with 2-(N,N-diisobutyl)acetamide as an alkyl appendage were designed, synthesized and tested for their ability to extract ruthenium selectively from nitric acid medium. The 2-(N,N-diisobutyl)acetamido ammonium iodide with two Pr and a Me substituents showed best results for the recovery of ruthenium. The optimized concentration of the solvent was 0.2 M in 30% isodecyl alc./n-dodecane. The stoichiometry of the complex was ascertained by slope anal. method and was 1:1 with respect to ligand L+I- and Ru(NO)(NO3)3. Ruthenium formed an adduct of structure LRu(NO)(NO3)3I in the extraction medium. Iodide ion played an important role in the formation of the stable and extractable complex of ruthenium. No extraction was observed when iodide was replaced by nitrate anion in the ligand. The ligand also showed good selectivity for ruthenium in the presence of other metal ions commonly found in nitric acid solutions of nuclear waste.

Journal of Hazardous Materials published new progress about Solvent extraction. 5326-82-9 belongs to class amides-buliding-blocks, and the molecular formula is C10H20ClNO, Formula: C10H20ClNO.

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Vu, Huu-Manh; Yong, Jia-Yuan; Chen, Fei-Wu; Li, Xu-Qin; Shi, Guo-Qing published the artcile< Rhodium-Catalyzed C(sp2)-H Amidation of Azine with Sulfonamides>, Synthetic Route of 1524-40-9, the main research area is regioselective rhodium catalyzed amidation azine sulfonamide.

Direct C-H amidation of azine with sulfonamide was developed for the first time. The reactions proceeded smoothly under benign conditions and gave the corresponding products with high selectivity. This approach shows high regioselectivity, wide substrate scope, and functional group tolerance. Addnl., this transformation can also be scaled up to the gram level. This strategy allows for the direct preparation of ortho-sulfonamide-substituted ketone products, thus providing a good complement to previous C-H amidation.

Journal of Organic Chemistry published new progress about Amidation. 1524-40-9 belongs to class amides-buliding-blocks, and the molecular formula is C6H6FNO2S, Synthetic Route of 1524-40-9.

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Shang, Chunliang; Li, Yuan; He, Tianhui; Liao, Yuandong; Du, Qiqiao; Wang, Pan; Qiao, Jie; Guo, Hongyan published the artcile< The prognostic miR-532-5p-correlated ceRNA-mediated lipid droplet accumulation drives nodal metastasis of cervical cancer>, Category: amides-buliding-blocks, the main research area is ACC1, Acetyl-CoA carboxylase 1; ACOX1, Acyl-CoA oxidase 1; ATCC, The American Type Culture Collection; BMI, Body mass index; CC, Cervical cancer; CPAT, The coding potential assessment tool; CPT1A, Carnitine palmitoyltransferase-1A; Cervical cancer; CircRNAs, circular RNAs; DFS, Disease free survival; EMT, Epithelial-mesenchymal transition; ES, enrichment score; FA, Fatty acid; FASN; FASN, Fatty acid synthase; GEPIA, The Gene Expression Profiling Interactive Analysis; GSEA, Gene set enrichment analysis; HE, Hematoxylin-eosin; HLECs, Human lymphatic endothelial cells; IHC, immunohistochemistry; ISH, In situ hybridization; LASSO, The least absolute shrinkage and selection operator; LDs, Lipid droplets; LINC01410, Long non-coding RNA 01410; LNM, lymph node metastasis; Lipid droplet; LncRNAs, Long noncoding RNAs; Lymph node metastasis; MES, Maximum enrichment score; MREs, MiRNA response elements; Mfe, The minimum free energy; NCF2, Neutrophil cytosolic factor 2; OS, Overall survival; PLIN2, Perilipin 2; PLs, Phospholipids; RIP, RNA immunoprecipitation; ROC, The receiver operating characteristic; SNR, signal-to-noise ratio; STR, short tandem repeat; TCGA, The Cancer Genome Atlas; TGs, Triglycerides; ceRNA, Competing endogenous RNA; miRNA prognostic model; miRNAs, MicroRNAs; ncRNAs, Noncoding RNAs.

The prognosis for cervical cancer (CC) patients with lymph node metastasis (LNM) is extremely poor. Lipid droplets (LDs) have a pivotal role in promoting tumor metastasis. The crosstalk mechanism between LDs and LNM modulated in CC remains largely unknown. This study aimed to construct a miRNA-dependent progonostic model for CC patients and investigate whether miR-532-5p has a biol. impact on LNM by regualting LDs accumulation. LASSO-Cox regression was applied to establish a prognostic prediction model. miR-532-5p had the lowest P-value in RNA expression (P < 0.001) and prognostic prediction (P < 0.0001) and was selected for further study. The functional role of the prognostic miR-532-5p-correlated competing endogenous RNA (ceRNA) network was investigated to clarify the crosstalk between LDs and LNM. The underlying mechanism was determined using site-directed mutagenesis, dual luciferase reporter assays, RNA immunoprecipitation assays, and rescue experiments A xenograft LNM model was established to evaluate the effect of miR-532-5p and orlistat combination therapy on tumor growth and LNM. A novel 5-miRNAs prognostic signature was constructed to better predict the prognosis of CC patient. Further study demonstrated that miR-532-5p inhibited epithelial-mesenchymal transition and lymphangiogenesis by regulating LDs accumulation. Interestingly, we also found that LDs accumulation promoted cell metastasis in vitro. Mechanistically, we demonstrated a miR-532-5p-correlated ceRNA network in which LINC01410 was bound directly to miR-532-5p and effectively functioned as miR-532-5p sponge to disinhibit its target gene-fatty acid synthase (FASN). Combined therapy with miR-532-5p and FASN inhibitor-orlistat further inhibited tumor growth and LNM in vivo. Our findings highlight a LD accumulation-dependent mechanism of miR-532-5p-modulated LNM and support treatment with miR-532-5p/orlistat as novel strategy for treating patients with LNM in CC. Journal of Advanced Research published new progress about 96829-58-2. 96829-58-2 belongs to class amides-buliding-blocks, and the molecular formula is C29H53NO5, Category: amides-buliding-blocks.

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Gorgojo-Martinez, Juan J.; Basagoiti-Carreno, Belen; Sanz-Velasco, Alberto; Serrano-Moreno, Clara; Almodovar-Ruiz, Francisca published the artcile< Effectiveness and tolerability of orlistat and liraglutide in patients with obesity in a real-world setting: The XENSOR Study>, Category: amides-buliding-blocks, the main research area is obesity cardiovascular risk factor orlistat liraglutide.

To evaluate in a real-world setting the effectiveness of two drugs, orlistat and liraglutide, in patients with overweight or obesity and insufficient weight loss (WL) after a lifestyle modification program. Retrospective, observational cohort study comparing clin. outcomes of orlistat 120 mg three times a day and liraglutide (up to 3 mg daily) in adult patients with BMI =30 kg/m2 or =27 kg/m2 with at least a weight-related comorbidity who had failed to lose at least 5% of their weight after 6 mo of lifestyle modification. The co-primary end-points, assessed at 3-6 mo and at the end of the follow-up, were weight change from baseline, proportion of patients who lost at least 5% of their baseline weight and adjusted differences in WL between both drugs. Five hundred patients, 400 in the group of orlistat (age 47.0, weight 107.8 kg) and 100 in the group of liraglutide (age 51.9 years, weight 105.1 kg), were included. WL with liraglutide (-7.7 kg) was significantly greater than that observed with orlistat (-3.3 kg), and more individuals lost at least 5% of their baseline weight with liraglutide (64.7%) than with orlistat (27.4%). In this real-world study, liraglutide showed a greater effectiveness in WL compared with orlistat and improved several obesity-associated metabolic and cardiovascular risk factors.

International Journal of Clinical Practice published new progress about Antiarrhythmics. 96829-58-2 belongs to class amides-buliding-blocks, and the molecular formula is C29H53NO5, Category: amides-buliding-blocks.

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Hedayatullah, Mir; Hugueny, Jean Claude published the artcile< Regioselective transfer of the sulfamoyl group on aminopyrimidines and -purines>, Application of C4H10N2O3S, the main research area is sulfamoylation aminopyrimidine aminopurine regioselectivity; pentachlorophenyl sulfamate sulfamoylating agent.

The regioselective transfer of the sulfamoyl group, to the exocyclic NH2 group of cytosine, thiamine, adenine and guanine is carried out in 86-98% yield by reaction with C6Cl5O3SNH2 in pyridine at 100°.

Phosphorus and Sulfur and the Related Elements published new progress about Regiochemistry. 25999-04-6 belongs to class amides-buliding-blocks, and the molecular formula is C4H10N2O3S, Application of C4H10N2O3S.

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Liu, Wei; Wu, Ge; Gao, Wenxia; Ding, Jinchang; Huang, Xiaobo; Liu, Miaochang; Wu, Huayue published the artcile< Palladium-catalyzed oxidative C=C bond cleavage with molecular oxygen: one-pot synthesis of quinazolinones from 2-amino benzamides and alkenes>, Synthetic Route of 5004-88-6, the main research area is aminobenzamide alkene palladium catalyst one pot tandem reaction; arylquinazolinone preparation green chem.

Palladium-catalyzed oxidative cleavage/cyclization was disclosed for the concise synthesis of various quinazolinone derivatives from readily available 2-aminobenzamides and terminal alkenes with excellent functional group tolerance. The synthetic features regarding the use of oxygen as a green oxidant and its utility were illustrated in the synthesis of sildenafil.

Organic Chemistry Frontiers published new progress about Anilines Role: RCT (Reactant), RACT (Reactant or Reagent). 5004-88-6 belongs to class amides-buliding-blocks, and the molecular formula is C9H12N2O3, Synthetic Route of 5004-88-6.

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Suleiman, Joseph Bagi; Nna, Victor Udo; Zakaria, Zaida; Othman, Zaidatul Akmal; Bakar, Ainul Bahiyah Abu; Mohamed, Mahaneem published the artcile< Obesity-induced testicular oxidative stress, inflammation and apoptosis: Protective and therapeutic effects of orlistat>, HPLC of Formula: 96829-58-2, the main research area is orlistat antiobesity agent testicular oxidative stress inflammation apoptosis obesity; Apoptosis; High-fat diet; Inflammation; Obesity; Orlistat; Oxidative stress.

The objective of this study was to determine effects of the anti-obesity drug orlistat, on testicular oxidative stress, inflammation and apoptosis in high-fat diet (HFD)-fed rats. Twenty-four adult male Sprague Dawley rats weighing 250-300 g were randomized into four groups (n = 6/group), namely; normal control (NC), high-fat diet (HFD), HFD plus orlistat (10 mg/kg body weight/day administered concurrently for 12 wk) (HFD + Opr) and HFD plus orlistat (10 mg/kg body weight/day administered 6 wk after induction of obesity) (HFD + Ot) groups. Antioxidant enzymes activities were significantly decreased, while mRNA levels of pro-apoptotic markers (p53, Bax/BCl-2, caspase-9, caspase-8 and caspase-3) were significantly increased in the testis of HFD group relative to NC group. Furthermore, mRNA levels of pro-inflammatory markers (nuclear factor kappa B, inducible nitric oxide synthase, tumor necrosis factor alpha and interleukin (IL)-1beta increased significantly, while anti-inflammatory marker (IL-10) decreased significantly in testis of the HFD group relative to NC group. However, in both models of orlistat intervention (protective and treatment models) up-regulated antioxidant enzymes, down-regulated inflammation and apoptosis were observed in the testis of HFD-fed rats. Orlistat ameliorated testicular dysfunction by attenuating oxidative stress, inflammation and apoptosis in HFD-fed rats, suggesting its potential protective and therapeutic effects in the testis compromised by obesity.

Reproductive Toxicology published new progress about Anti-inflammatory agents. 96829-58-2 belongs to class amides-buliding-blocks, and the molecular formula is C29H53NO5, HPLC of Formula: 96829-58-2.

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics