Category: amides-buliding-blocks

On April 30, 2015, Schantz, Michele M.; Benner, Bruce A. Jr.; Heckert, N. Alan; Sander, Lane C.; Sharpless, Katherine E.; Vander Pol, Stacy S.; Vasquez, Y.; Villegas, M.; Wise, Stephen A.; Alwis, K. Udeni; Blount, Benjamin C.; Calafat, Antonia M.; Li, Zheng; Silva, Manori J.; Ye, Xiaoyun; Gaudreau, Eric; Patterson, Donald G. Jr.; Sjodin, Andreas published an article.Formula: C10H11NO3 The title of the article was Development of urine standard reference materials for metabolites of organic chemicals including polycyclic aromatic hydrocarbons, phthalates, phenols, parabens, and volatile organic compounds. And the article contained the following:

Two new Standard Reference Materials (SRMs), SRM 3672 Organic Contaminants in Smokers’ Urine (Frozen) and SRM 3673 Organic Contaminants in Non-Smokers’ Urine (Frozen), have been developed in support of studies for assessment of human exposure to select organic environmental contaminants. Collaborations among three organizations resulted in certified values for 11 hydroxylated polycyclic aromatic hydrocarbons (OH-PAHs) and reference values for 11 phthalate metabolites, 8 environmental phenols and parabens, and 24 volatile organic compound (VOC) metabolites. Reference values are also available for creatinine and the free forms of caffeine, theobromine, ibuprofen, nicotine, cotinine, and 3-hydroxycotinine. These are the first urine Certified Reference Materials characterized for metabolites of organic environmental contaminants. Noteworthy, the mass fractions of the environmental organic contaminants in the two SRMs are within the ranges reported in population survey studies such as the National Health and Nutrition Examination Survey (NHANES) and the Canadian Health Measures Survey (CHMS). These SRMs will be useful as quality control samples for ensuring compatibility of results among population survey studies and will fill a void to assess the accuracy of anal. methods used in studies monitoring human exposure to these organic environmental contaminants. The experimental process involved the reaction of 2-(4-Methylbenzamido)acetic acid(cas: 27115-50-0).Formula: C10H11NO3

The Article related to urine metabolite pah phthalate phenol paraben volatile standard reference, Toxicology: Methods (Including Analysis) and other aspects.Formula: C10H11NO3

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Yamamoto, Priscila A.; de Moraes, Natalia V.; Silva, Vinicius P.; Lepera, Jose S. published an article in 2016, the title of the article was Simultaneous analysis of biological indicators of exposure to ethylbenzene, styrene, toluene and xylene solvents in urine by HPLC-UV.Recommanded Product: 2-(4-Methylbenzamido)acetic acid And the article contains the following content:

A method for simultaneous anal. of phenylglyoxylic (PGA), mandelic (MA), hippuric (HA), ortho-, meta- and para-methylhippuric (o-, m- and p-MHA) acids in urine, by high performance liquid chromatog. with UV detection was developed and validated. The substances are biotransformation products used as biol. indicators to assess the exposure of workers, resp., to ethylbenzene, styrene, toluene and xylene. The urinary metabolites were separated using a C18 column and a mixture of 5 mM phosphate buffer pH 2.6:acetonitrile (92:8, volume/volume) as mobile phase. Urine samples were extracted with dichloromethane:ethyl acetate (70:30, volume/volume). The method used phenacetin as internal standard and was linear in the interval of 100-500 μg mL-1 for PGA and MA and 150-700 μg mL-1 for HA and MHA. The detection limits in μg mL-1, were 19.8 for PGA, 1.7 for MA, 4.1 for HA, 3.9 for o-MHA, 3.3 for m-MHA and 7.3 for p-MHA. Intra- and inter-assay precisions (as relative standard deviation) were all less than 15% and accuracy (as relative standard error) did not exceed 12.3%. The recovery was higher than 65% for all metabolites. The developed method will be applied to biol. evaluation of workers exposure to these solvents. The experimental process involved the reaction of 2-(4-Methylbenzamido)acetic acid(cas: 27115-50-0).Recommanded Product: 2-(4-Methylbenzamido)acetic acid

The Article related to volatile organic compound exposure urine analysis hplc uv, Toxicology: Methods (Including Analysis) and other aspects.Recommanded Product: 2-(4-Methylbenzamido)acetic acid

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

On July 15, 2013, Gagne, Sebastien published an article.Reference of 2-(4-Methylbenzamido)acetic acid The title of the article was Achieving greater selectivity for the analysis of o-, m-, p-methylhippuric acids in workers’ urine by ultra performance liquid chromatography coupled with tandem mass spectrometry. And the article contained the following:

A selective method analyzing sep. o-, m- and p-methylhippuric acid isomers in workers’ urine samples has been developed using ultra performance liquid chromatog. coupled with tandem mass spectrometry. Chromatog. separation has been optimized to resolve the three isomers at baseline. Combined with this optimal separation, the mass spectrometer allowed rapid switching from MRM scan to full scan and product ion scan within the chromatog. peak. This feature allowed the retention of analyte chem. structure information for the three methylhippuric acid isomers in parallel with the simultaneous acquisition of quant. data. Such an approach is unequaled for the reliability of the data generated and it can be applied to each isomer sep. The method was adjusted to a dynamic range between 0.2 mM and 8.12 mM for o-methylhippuric acid and p-methylhippuric acid, and between 0.41 mM and 16.23 mM for m-methylhippuric acid to cover the biol. exposure index. A negligible matrix effect was observed with the conditions used. Also, intra-day and inter-day precisions were both <6% for all the concentration levels tested and the accuracy was evaluated at 97%. The inclusion of simultaneous full scan acquisitions did not prevent the robustness of the quant. data. The method applied to the determination of inter-laboratory proficient test samples led to results in the tolerated range. Moreover, urine samples from workers were robustly quantified and the MHA levels were all below the biol. exposure index reference value. The experimental process involved the reaction of 2-(4-Methylbenzamido)acetic acid(cas: 27115-50-0).Reference of 2-(4-Methylbenzamido)acetic acid

The Article related to xylene exposure methylhippurate urine analysis worker, Toxicology: Methods (Including Analysis) and other aspects.Reference of 2-(4-Methylbenzamido)acetic acid

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

On December 31, 2014, Meyer-Monath, Marie; Chatellier, Claudine; Rouget, Florence; Morel, Isabelle; Lestremau, Francois published an article.Reference of 2-(4-Methylbenzamido)acetic acid The title of the article was Development of a multi-residue method in a fetal matrix: analysis of meconium. And the article contained the following:

Meconium is the earliest stool of newborns. It is a complex matrix that reflects the degree of fetal exposure to environmental pollutants. To investigate exposure to xenobiotics, an anal. method was developed to identify and quantify some pesticides and their metabolites and BTEX metabolites in meconium. Samples were prepared by two liquid-solid extractions and purified twice using SPE cartridges, followed by anal. with liquid chromatog. coupled with tandem mass spectrometry. SPE cartridges (polymeric phase with hydrophilic and hydrophobic interactions, ion exchange, mixed mode) were tested and matrix effects were evaluated to determine purification performance. The quantification limits in meconium of this multi-residue method were in the range of 30 ng g-1. The anal. method was applied to “real” meconium samples. Some target analytes were determined in most samples. The experimental process involved the reaction of 2-(4-Methylbenzamido)acetic acid(cas: 27115-50-0).Reference of 2-(4-Methylbenzamido)acetic acid

The Article related to pollutant multiresidue analysis fetus meconium lc ms, Toxicology: Methods (Including Analysis) and other aspects.Reference of 2-(4-Methylbenzamido)acetic acid

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

On October 31, 2012, Alwis, K. Udeni; Blount, Benjamin C.; Britt, April S.; Patel, Dhrusti; Ashley, David L. published an article.Category: amides-buliding-blocks The title of the article was Simultaneous analysis of 28 urinary VOC metabolites using ultra high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (UPLC-ESI/MSMS). And the article contained the following:

Volatile organic compounds (VOCs) are ubiquitous in the environment, originating from many different natural and anthropogenic sources, including tobacco smoke. Long-term exposure to certain VOCs may increase the risk for cancer, birth defects, and neurocognitive impairment. Therefore, VOC exposure is an area of significant public health concern. Urinary VOC metabolites are useful biomarkers for assessing VOC exposure because of non-invasiveness of sampling and longer physiol. half-lives of urinary metabolites compared with VOCs in blood and breath. We developed a method using reversed-phase ultra high performance liquid chromatog. (UPLC) coupled with electrospray ionization tandem mass spectrometry (ESI/MSMS) to simultaneously quantify 28 urinary VOC metabolites as biomarkers of exposure. We describe a method that monitors metabolites of acrolein, acrylamide, acrylonitrile, benzene, 1-bromopropane, 1,3-butadiene, carbon-disulfide, crotonaldehyde, cyanide, N,N-dimethylformamide, ethylbenzene, ethylene oxide, propylene oxide, styrene, tetrachloroethylene, toluene, trichloroethylene, vinyl chloride and xylene. The method is accurate (mean accuracy for spiked matrix ranged from 84 to104%), sensitive (limit of detection ranged from 0.5 to 20 ng mL-1) and precise (the relative standard deviations ranged from 2.5 to 11%). We applied this method to urine samples collected from 1203 non-smokers and 347 smokers and demonstrated that smokers have significantly elevated levels of tobacco-related biomarkers compared to non-smokers. We found significant (p < 0.0001) correlations between serum cotinine and most of the tobacco-related biomarkers measured. These findings confirm that this method can effectively quantify urinary VOC metabolites in a population exposed to volatile organics The experimental process involved the reaction of 2-(4-Methylbenzamido)acetic acid(cas: 27115-50-0).Category: amides-buliding-blocks

The Article related to urine voc metabolite uplc ms tobacco smoke biomarker, Toxicology: Methods (Including Analysis) and other aspects.Category: amides-buliding-blocks

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

On December 29, 2017, Lunn, Daniel B.; Yun, Young J.; Jorgenson, James W. published an article.Product Details of 27115-50-0 The title of the article was Retention and effective diffusion of model metabolites on porous graphitic carbon. And the article contained the following:

The study of metabolites in biol. samples is of high interest for a wide range of biol. and pharmaceutical applications. Reversed phase liquid chromatog. is a common technique used for the separation of metabolites, but it provides little retention for polar metabolites. An alternative to C18 bonded phases, porous graphitic carbon has the ability to provide significant retention for both non-polar and polar analytes. The goal of this work is to study the retention and effective diffusion properties of porous graphitic carbon, to see if it is suitable for the wide injection bands and long run times associated with long, packed capillary-scale separations The retention of a set of standard metabolites was studied for both stationary phases over a wide range of mobile phase conditions. Porous graphitic carbon benefits from significantly increased retention (often >100 fold) under initial gradient conditions for these metabolites, suggesting much improved ability to focus a wide injection band at the column inlet. The effective diffusion properties of these columns were studied using peak-parking experiments with the standard metabolites under a wide range of retention conditions. Under the high retention conditions, which can be associated with retention after injection loading for gradient separations, Deff/Dm ∼ 0.1 for both the C18-bonded and porous graphitic carbon columns. As C18 bonded particles are widely, and successfully used for long gradient separations without issue of increasing peak width from longitudinal diffusion, this suggests that porous graphitic carbon should be amenable for long runtime gradient separations as well. The experimental process involved the reaction of 2-(4-Methylbenzamido)acetic acid(cas: 27115-50-0).Product Details of 27115-50-0

The Article related to metabolite retention diffusion porous graphitic carbon chromatog, effective diffusion, metabolites, porous graphitic carbon, sample pre-concentration, Biochemical Methods: Chromatographic and other aspects.Product Details of 27115-50-0

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Tinland, Bernard published an article in 1973, the title of the article was Theoretical EHT [extended Hueckel theory] study of structure-activity relations in a series of sulfonamides.Electric Literature of 97-09-6 And the article contains the following content:

The carbonic anhydrase inhibitory activities of 17 sulfonamides were compared with their structures, based on quantum chem. calculations performed within the framework of the extended Hueckel theory. Correlations were found between the activity of mols. and the reduced Mulliken overlap populations relative to the SO2 group. The experimental process involved the reaction of 3-Nitro-4-chlorobenzenesulfonamide(cas: 97-09-6).Electric Literature of 97-09-6

The Article related to sulfonamide structure activity, quantum chem drug activity, carbonic anhydrase sulfonamide, Pharmacodynamics: Structure-Activity and other aspects.Electric Literature of 97-09-6

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Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

Ghamari, Farhad; Bahrami, Abdulrahman; Yamini, Yadollah; Shahna, Farshid Ghorbani; Moghimbeigi, Abbas published an article in 2017, the title of the article was Hollow-fiber liquid-phase microextraction based on carrier-mediated transport for determination of urinary methyl hippuric acids.Reference of 2-(4-Methylbenzamido)acetic acid And the article contains the following content:

Hollow-fiber liquid-phase microextraction based on carrier-mediated transport for anal. of Me hippuric acids in aqueous samples is described. Under optimized conditions, relative recoveries of 83%-98% and limits of detection of 2-3μg L-1 were obtained, with linear calibration curves for the three isomers in the range of 10-50,000μg L-1. The method was applied for the determination of urine samples of volunteers at various working environments. The experimental process involved the reaction of 2-(4-Methylbenzamido)acetic acid(cas: 27115-50-0).Reference of 2-(4-Methylbenzamido)acetic acid

The Article related to urinary methyl hippuric acid microextraction, Biochemical Methods: Chromatographic and other aspects.Reference of 2-(4-Methylbenzamido)acetic acid

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

On March 20, 2013, Liu, Jie; Mei, Yong; Wang, Ya-nan; Gu, Ying-ying; Wang, Qian; Jiang, Jin-feng published an article.Category: amides-buliding-blocks The title of the article was Standardized study on determination of methylhippuric acids in urine by HPLC. And the article contained the following:

The aim of this paper is to get a better separation of methylhippuric acid isomers and establish a standardized method for the determination of methylhippuric acids in urine by HPLC. C18 column was used to determine the methylhippuric acid. The mobile phase was methanol-0.1% acetic acid (33:67). The detector was performed at λ=230 nm, at 0.7 mVmin. Calibration curves of three methylhippuric acids were linear in the range of 25μg/mL∼1000μg/mL, with a correlation coefficient more than 0.999. The average recovery was in the range of 91.36%∼101.36% and the average RSD was in the range of 2.0%∼9.6%. The min. detection concentrations of 2-MHA, 3-MHA, 4-MHA were resp. 0.47μg/mL, 0.46μg/mL, 0.41μg/mL. The separation of 4-methylhippuric acid and 3-methylhippuric acid was up to 1.44. The method is able to make 3-methylhippuric acid and 4-methylhippuric acid baseline separated, and the methodol. indicators are in line with the relevant requirements of the method for determination of chems. in the occupational health standard-setting Guide Part V. So it can be used to determine methylhippuric acids in urine of xylene exposure workers. The experimental process involved the reaction of 2-(4-Methylbenzamido)acetic acid(cas: 27115-50-0).Category: amides-buliding-blocks

The Article related to hplc methylhippuric acid determination, Biochemical Methods: Chromatographic and other aspects.Category: amides-buliding-blocks

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics

On May 15, 2022, Sun, Feifei; Tan, Haiguang; Abdallah, Mohamed F.; Li, Yanshen; Zhou, Jinhui; Li, Yi; Yang, Shupeng published an article.Name: N-((4-Aminophenyl)sulfonyl)acetamide The title of the article was A novel calibration strategy based on isotopic distribution for high-throughput quantitative analysis of pesticides and veterinary drugs using LC-HRMS. And the article contained the following:

Preparation of calibration curves is a critical step for large-scale quantification. However, this procedure is time-consuming, labor intensive. Herein, a novel isotopologue multipoint calibration (IMC) strategy, was proposed and demonstrated for the simultaneous quantitation of 120 pesticides and 83 veterinary drugs in surface water samples using Liquid Chromatog.-High Resolution Mass Spectrometry (LC-HRMS). In this strategy, the natural isotopic distribution was used to generate external calibration curves, eliminating the need of analyst’s adjustment and many sets of chem. standard solutions required in external calibration curves. Addnl., this strategy was comprehensively validated, and the results indicated this strategy had better performance in both accuracy and precision, fully meeting the requirements for the quant. anal. Interestingly, for the samples with high concentration beyond the upper limit of quantitation, the IMC strategy could avoid samples dilution by monitoring the less abundant isotopic channels. Furthermore, the IMC method was successfully applied in the surface water samples collected from Anhui province, China. Among which, sulfamethoxazole and imidacoprid were the main contributors. In conclusion, we present a promising LC-HRMS strategy for the accurate quantitation of small mols., which has a potential application in food and environmental anal. The experimental process involved the reaction of N-((4-Aminophenyl)sulfonyl)acetamide(cas: 144-80-9).Name: N-((4-Aminophenyl)sulfonyl)acetamide

The Article related to pesticide veterinary drug isotopic distribution calibration, high resolution mass spectrometry, isotope distribution, quantitative analysis, water environment pollution, Waste Treatment and Disposal: Other and other aspects.Name: N-((4-Aminophenyl)sulfonyl)acetamide

Referemce:
Amide – Wikipedia,
Amide – an overview | ScienceDirect Topics